University College London (UCL) Centre for Advanced Biomedical Imaging, Division of Medicine, UCL, London, UK; Centre for Mathematics and Physics in the Life Sciences and Experimental Biology (CoMPLEX), UCL, London, UK.
Trends Genet. 2013 Dec;29(12):700-11. doi: 10.1016/j.tig.2013.08.004. Epub 2013 Sep 12.
The immense challenge of annotating the entire mouse genome has stimulated the development of cutting-edge imaging technologies in a drive for novel information. These techniques promise to improve understanding of the genes involved in embryo development, at least one third of which have been shown to be essential. Aligning advanced imaging technologies with biological needs will be fundamental to maximising the number of phenotypes discovered in the coming years. International efforts are underway to meet this challenge through an integrated and sophisticated approach to embryo phenotyping. We review rapid advances made in the imaging field over the past decade and provide a comprehensive examination of the relative merits of current and emerging techniques. The aim of this review is to provide a guide to state-of-the-art embryo imaging that will enable informed decisions as to which technology to use and fuel conversations between expert imaging laboratories, researchers, and core mouse production facilities.
注释整个小鼠基因组的巨大挑战刺激了前沿成像技术的发展，以获取新的信息。这些技术有望提高对胚胎发育相关基因的理解，至少有三分之一的基因已被证明是必不可少的。将先进的成像技术与生物需求相结合，对于最大限度地发现未来几年的表型至关重要。国际社会正在通过综合和复杂的胚胎表型方法来应对这一挑战。我们回顾了过去十年成像领域的快速进展，并全面考察了当前和新兴技术的相对优势。本篇综述的目的是提供胚胎成像的最新技术指南，以便就使用哪种技术做出明智决策，并促进专家成像实验室、研究人员和核心小鼠生产机构之间的对话。
