Escola Superior de Saúde Dr. Lopes Dias, Instituto Politécnico de Castelo Branco, Campus da Talagueira, 6000-767 Castelo Branco, Portugal; Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Unidade de Parasitologia e Microbiologia Médicas (UPMM), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
Vet Parasitol. 2013 Nov 15;198(1-2):235-9. doi: 10.1016/j.vetpar.2013.08.021. Epub 2013 Aug 29.
Although cystic echinococcosis (CE) has been a recognized public health problem in Portugal, molecular data regarding the types and prevalence of infecting strains of its etiological agent (Echinococcus granulosus) are still scarce. Over the last years we have been evaluating the prevalence of CE in the country, and in this report we determined the parasite genotypes infecting sheep, goats, cattle and human in Portugal, based on 209 hydatid cysts recovered from liver (n=96), lung (n=95), pancreas (n=17) and kidney (n=1) samples obtained between 2008 and 2011. Protoscoleces or germinal layers were collected from individual cysts, DNA was extracted, and a part of the mitochondrial DNA encoding the cytochrome c oxidase subunit 1 was amplified by PCR. Overall, the results confirm the overall dominance of the G1-G3 cluster of strains, which are particularly prevalent in southern Portugal in livestock ruminants. Unexpectedly, one parasite sequence with cattle origin was found to correspond to E. granulosus G7 genotype (also known as E. intermedius), here reported for the first time in bovine, in Portugal.
虽然囊性包虫病（CE）在葡萄牙已被确认为公共卫生问题，但有关其病原体（细粒棘球绦虫）感染株的类型和流行率的分子数据仍然很少。在过去几年中，我们一直在评估葡萄牙的 CE 流行率，在本报告中，我们根据 2008 年至 2011 年间从肝脏（n=96）、肺脏（n=95）、胰腺（n=17）和肾脏（n=1）样本中获得的 209 个包虫囊，确定了感染葡萄牙绵羊、山羊、牛和人类的寄生虫基因型。从单个包虫囊中收集Protoscoleces 或生殖层，提取 DNA，并通过 PCR 扩增编码细胞色素 c 氧化酶亚基 1 的线粒体 DNA 的一部分。总体而言，结果证实了 G1-G3 群菌株的总体优势，这些菌株在葡萄牙南部的牲畜反刍动物中特别流行。出乎意料的是，发现一个源自牛的寄生虫序列与 E. granulosus G7 基因型（也称为 E. intermedius）相对应，这是首次在葡萄牙的牛中报道。
