Sałamatin Rusłan, Kowal Jerzy, Nosal Paweł, Kornaś Sławomir, Cielecka Danuta, Jańczak Dawid, Patkowski Waldemar, Gawor Jakub, Kornyushin Vadim, Golab Elzbieta, Šnábel Viliam
Department of General Biology and Parasitology, Medical University of Warsaw, Chałubińskiego 5, 02-004, Warsaw, Poland.
Department of Parasitology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791, Warsaw, Poland.
Parasitol Res. 2017 Nov;116(11):3077-3085. doi: 10.1007/s00436-017-5618-4. Epub 2017 Oct 3.
Cystic echinococcosis is one of the most important zoonotic diseases affecting humans and livestock worldwide, and is endemic in Poland. A set of six isolates on larval stages of Echinococcus granulosus sensu lato tapeworms collected from three humans, two pigs and one sheep from Polish foci of CE was examined by DNA sequencing of two mitochondrial genes (cox1, rrnS). The results demonstrated the presence of E. canadensis and E. granulosus sensu stricto in the investigated hydatid cysts. The former species was found in all five isolates from pigs and humans derived from central Poland. In a sheep hydatid cyst originating from Lesser Poland Voivodeship in southern Poland, E. granulosus s. s. (G1 genotype) was identified. This is the first report of an unambiguously autochthonous infection with E. granulosus s. s. in Poland. The global distribution and host affiliations of the commonly occurring G1 microvariant with nucleotide change 56C/T in cox1, detected here in Polish sheep, are discussed. The finding that sheep harboured E. granulosus s. s. may have important consequences for developing effective hydatid control programmes in Poland due to its longer maturation rate in dogs compared with E. canadensis G7. This may lead to greater expenditures for purchasing anthelmintics to provide an appropriate dosing regime in sheep-raising areas of the country.
囊型棘球蚴病是全球影响人类和牲畜的最重要的人畜共患病之一，在波兰呈地方性流行。对从波兰囊型棘球蚴病疫源地的3名人类、2头猪和1只绵羊采集的6株细粒棘球绦虫幼虫阶段的分离株，通过两个线粒体基因（cox1、rrnS）的DNA测序进行了检测。结果表明，在所调查的包虫囊肿中存在加拿大棘球绦虫和狭义细粒棘球绦虫。前一种在来自波兰中部的猪和人类的所有5个分离株中均有发现。在源自波兰南部小波兰省的一个羊包虫囊肿中，鉴定出狭义细粒棘球绦虫（G1基因型）。这是波兰首次明确报告本土发生狭义细粒棘球绦虫感染。讨论了此处检测到的波兰绵羊中常见的cox1基因核苷酸变化56C/T的G1微小变异体的全球分布和宿主归属。绵羊携带狭义细粒棘球绦虫这一发现，可能对波兰制定有效的包虫控制计划产生重要影响，因为与加拿大棘球绦虫G7相比，它在犬体内的成熟速度更长。这可能导致在该国养羊地区购买驱虫药以提供适当给药方案的费用增加。
