一种用于检测生物体液和细胞分泌物中抗菌肽的多重分析方法。
A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions.
机构信息
Thayer School of Engineering, Dartmouth College, Hanover, NH, USA.
出版信息
J Immunol Methods. 2013 Nov 29;397(1-2):71-6. doi: 10.1016/j.jim.2013.09.001. Epub 2013 Sep 11.
Abstract
Mucosal tissues represent the front line in defense against potential pathogens, and one means by which mucosa provide protection is via the secretion of antimicrobials which can interfere with potential pathogens as well as recruit and modify the responses of immune cells. Here we describe adaptation of ELISA assays to microsphere format, facilitating simultaneous quantification of antimicrobial peptides including elafin, MIP3α, HBD2, HBD3, SLPI, RANTES, SDF1, lactoferrin, LL-37, and HNP1-3. The multiplexed assay exhibits excellent reproducibility, shows linearity over a two order of magnitude concentration range for most analytes, is compatible with biological fluids such as cervicovaginal lavage fluid, and presents significant cost and sample savings relative to traditional ELISA assays.
摘要
黏膜组织是抵御潜在病原体的第一道防线，黏膜提供保护的一种方式是分泌抗菌肽，抗菌肽可以干扰潜在的病原体，并招募和调节免疫细胞的反应。在这里，我们描述了 ELISA 检测方法向微球格式的适应性改造，这有助于同时定量分析抗菌肽，包括 Elafin、MIP3α、HBD2、HBD3、SLPI、RANTES、SDF1、乳铁蛋白、LL-37 和 HNP1-3。这种多重检测方法具有出色的重现性，对于大多数分析物，在两个数量级的浓度范围内呈线性关系，与生物体液（如宫颈阴道灌洗液）兼容，与传统 ELISA 检测相比，具有显著的成本和样本节省。
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