Thijs Willemien, Janssen Kirsten, van Schadewijk Annemarie M, Papapoulos Socrates E, le Cessie Saskia, Middeldorp Saskia, Melissant Christian F, Rabe Klaus F, Hiemstra Pieter S
Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands.
Department of Pulmonology, Spaarne Hospital, Hoofddorp, The Netherlands.
PLoS One. 2015 Nov 6;10(11):e0140986. doi: 10.1371/journal.pone.0140986. eCollection 2015.
Allergy is often accompanied by infections and lower levels of antimicrobial peptides (AMPs). Vitamin D has been shown to increase expression of selected AMPs. In this study we investigated whether antimicrobial peptide levels in nasal secretions of allergic asthma patients are lower than in healthy controls, and whether administration of the active form of vitamin D (1,25(OH)2D3) affects these antimicrobial peptide levels.
The levels of antimicrobial peptides in nasal secretions were compared between 19 allergic asthma patients and 23 healthy controls. The effect of seven days daily oral treatment with 2 μg 1,25(OH)2D3 on antimicrobial peptides in nasal secretions was assessed in a placebo-controlled cross-over clinical study.
Levels of neutrophil α-defensins (human neutrophil peptides 1-3; HNP1-3) and lipocalin 2 (LCN2; also known as NGAL) were significantly lower in asthmatics, but no differences in LL-37 and SLPI were detected. Treatment with a short-term 1,25(OH)2D3 caused a small increase in HNP1-3, but not when the asthma and control groups were analyzed separately. LL-37, LCN2 and SLPI did not change after treatment with 1,25(OH)2D3.
Levels of the antimicrobial peptides HNP1-3 and LCN2 are lower in nasal secretions in asthmatics and are not substantially affected by a short-term treatment with active vitamin D.
过敏常伴有感染及抗菌肽（AMPs）水平降低。维生素D已被证明可增加特定抗菌肽的表达。在本研究中，我们调查了过敏性哮喘患者鼻分泌物中的抗菌肽水平是否低于健康对照者，以及活性形式的维生素D（1,25(OH)2D3）给药是否会影响这些抗菌肽水平。
比较了19名过敏性哮喘患者和23名健康对照者鼻分泌物中抗菌肽的水平。在一项安慰剂对照的交叉临床研究中，评估了每日口服2μg 1,25(OH)2D3连续7天对鼻分泌物中抗菌肽的影响。
哮喘患者中性粒细胞α-防御素（人类中性粒细胞肽1-3；HNP1-3）和脂质运载蛋白2（LCN2；也称为NGAL）的水平显著较低，但未检测到LL-37和分泌性白细胞蛋白酶抑制因子（SLPI）有差异。短期使用1,25(OH)2D3治疗使HNP1-3略有增加，但对哮喘组和对照组分别分析时未出现此情况。1,25(OH)2D3治疗后LL-37、LCN2和SLPI未发生变化。
哮喘患者鼻分泌物中抗菌肽HNP1-3和LCN2的水平较低，且活性维生素D的短期治疗对其影响不大。
