Tianjin Medical University General Hospital, Tianjin Geriatrics Institute, Department of Health Care Medicine, Tianjin,, China.
Clinics (Sao Paulo). 2013;68(8):1084-8. doi: 10.6061/clinics/2013(08)04.
The onset of chronic subdural hematoma may be associated with direct or indirect minor injuries to the head or a poorly repaired vascular injury. Endothelial progenitor cells happen to be one of the key factors involved in hemostasis and vascular repair. This study was designed to observe the levels of endothelial progenitor cells, white blood cells, platelets, and other indicators in the peripheral blood of patients diagnosed with chronic subdural hematoma to determine the possible relationship between the endothelial progenitor cells and the occurrence, development, and outcomes of chronic subdural hematoma.
We enrolled 30 patients with diagnosed chronic subdural hematoma by computer tomography scanning and operating procedure at Tianjin Medical University General Hospital from July 2009 to July 2011. Meanwhile, we collected 30 cases of peripheral blood samples from healthy volunteers over the age of 50. Approximately 2 ml of blood was taken from veins of the elbow to test the peripheral blood routine and coagulation function. The content of endothelial progenitor cells in peripheral blood mononuclear cells was determined by flow cytometry.
The level of endothelial progenitor cells in peripheral blood was significantly lower in preoperational patients with chronic subdural hematomas than in controls. There were no significant differences between the two groups regarding the blood routine and coagulation function. However, the levels of circulating endothelial progenitor cells were significantly different between the recurrent group and the non-recurrent group.
The level of circulating endothelial progenitor cells in chronic subdural hematoma patients was significantly lower than the level in healthy controls. Meanwhile, the level of endothelial progenitor cells in recurrent patients was significantly lower than the level in patients without recurrence. Endothelial progenitor cells may be related to the occurrence and recurrence of chronic subdural hematoma.
慢性硬脑膜下血肿的发病可能与头部直接或间接的轻微损伤或血管修复不良有关。内皮祖细胞恰好是参与止血和血管修复的关键因素之一。本研究旨在观察诊断为慢性硬脑膜下血肿患者外周血中内皮祖细胞、白细胞、血小板等指标的水平，以确定内皮祖细胞与慢性硬脑膜下血肿的发生、发展和转归之间的可能关系。
我们通过计算机断层扫描和天津医科大学总医院手术程序，于 2009 年 7 月至 2011 年 7 月期间招募了 30 名诊断为慢性硬脑膜下血肿的患者。同时，我们从 50 岁以上的健康志愿者中采集了 30 例外周血样本。从肘静脉抽取约 2ml 血液，检测外周血常规和凝血功能。通过流式细胞术测定外周血单个核细胞中内皮祖细胞的含量。
术前慢性硬脑膜下血肿患者外周血内皮祖细胞水平明显低于对照组。两组血常规和凝血功能无显著差异。然而，复发组和非复发组之间循环内皮祖细胞水平有显著差异。
慢性硬脑膜下血肿患者外周血循环内皮祖细胞水平明显低于健康对照组。同时，复发患者的内皮祖细胞水平明显低于无复发患者。内皮祖细胞可能与慢性硬脑膜下血肿的发生和复发有关。
