University of Agricultural Sciences and Veterinary Medicine, Mănăştur Street, 3-5, 400372, Cluj-Napoca, Romania.
Phytochem Anal. 2013 Sep-Oct;24(5):484-92. doi: 10.1002/pca.2460.
Sea buckthorn (Hippophaë rhamnoides L.) is known to be rich in many bioactive compounds (such as vitamins, phenolics, carotenoids) important for human health and nutrition. Among the phenolics, berries and leaves contain a wide range of flavonols that are good quality and authenticity biomarkers.
To compare the composition of the main flavonols of Romanian sea buckthorn berry and leaf varieties and to identify the specific biomarkers that contribute to sample differentiation among varieties.
Six varieties of cultivated sea buckthorn (ssp. Carpatica) berries and leaves were analysed by UHPLC/PDA-ESI/MS.
Berries and leaves contained mainly isorhamnetin (I) glycosides in different ratios. Whereas I-3-neohesperidoside, I-3-glucoside, I-3-rhamnosylglucoside, I-3-sophoroside-7-rhamnoside and free isorhamnetin were predominant for berries (out of 17 compounds identified), I-3-rhamnosylglucoside, I-3-neohesperidoside, I-3-glucoside, quercetin-3-pentoside, kaempferol-3-rutinoside, and quercetin-3-glucoside were predominant in leaves (out of 19 compounds identified). Berries contained, on average, 917 mg/100 g DW flavonol glycosides. Leaves had higher content of flavonol glycosides than berries, on average 1118 mg/100 g DW. The variation of the quantitative dataset analysed using principal component analysis accounted for 91% of the total variance in the case of berries and 73% in case of leaves, demonstrating a good discrimination among samples.
Based on quantitative analysis, by principal component analysis, the flavonol derivatives can be considered as biomarkers to discriminate among varieties and to recognise specifically the berry versus leaf composition.
沙棘（ Hippophaë rhamnoides L.）被认为富含多种对人体健康和营养至关重要的生物活性化合物（如维生素、酚类、类胡萝卜素）。在酚类化合物中，浆果和叶子含有广泛的类黄酮，它们是高质量和真实性的生物标志物。
比较罗马尼亚沙棘浆果和叶子品种中主要类黄酮的组成，并确定有助于品种间样品区分的特定生物标志物。
采用 UHPLC/PDA-ESI/MS 对 6 种栽培沙棘（ ssp. Carpatica）浆果和叶子进行分析。
浆果和叶子中主要以不同比例的异鼠李素（I）糖苷形式存在。而 I-3-新橙皮糖苷、I-3-葡萄糖苷、I-3-鼠李糖苷、I-3-槐糖苷-7-鼠李糖苷和游离异鼠李素是浆果中主要的成分（在所鉴定的 17 种化合物中），I-3-鼠李糖苷、I-3-新橙皮糖苷、I-3-葡萄糖苷、槲皮素-3-戊糖苷、山奈酚-3-鼠李糖苷和槲皮素-3-葡萄糖苷是叶子中主要的成分（在所鉴定的 19 种化合物中）。浆果中平均含有 917mg/100g DW 的类黄酮糖苷。叶子中的类黄酮糖苷含量高于浆果，平均为 1118mg/100g DW。采用主成分分析对定量数据集进行分析，在浆果中解释了 91%的总方差，在叶子中解释了 73%的总方差，证明了样品之间的良好区分。
基于定量分析，通过主成分分析，可以将类黄酮衍生物视为区分品种和识别浆果与叶子成分的生物标志物。
