Division of Nutritional Sciences and Department of Animal Sciences, University of Illinois, Urbana, Illinois, United States of America.
PLoS One. 2013 Sep 11;8(9):e74998. doi: 10.1371/journal.pone.0074998. eCollection 2013.
Cooked bean powders are a promising novel protein and fiber source for dogs, which have demonstrated potential to alter microbial composition and function for chronic disease control and prevention. This study aimed to determine the impact of cooked navy bean powder fed as a staple food ingredient on the fecal microbiome of healthy adult pet dogs.
METHODOLOGY/PRINCIPAL FINDINGS: Fecal samples from healthy dogs prior to dietary control and after 4 wk of dietary treatment with macro- and micronutrient matched diets containing either 0 or 25% cooked navy beans (n = 11 and n = 10, respectively) were analyzed by 454-pyrosequencing of the 16S rRNA gene. There were few differences between dogs fed the control and navy bean diets after 4 wk of treatment. These data indicate that there were no major effects of navy bean inclusion on microbial populations. However, significant differences due to dietary intervention onto both research diets were observed (i.e., microbial populations at baseline versus 4 wk of intervention with 0 or 25% navy bean diets). After 4 wk of dietary intervention on either control or navy bean diet, the Phylum Firmicutes was increased and the Phyla Actinobacteria and Fusobacteria were decreased compared to baseline.
No negative alterations of microbial populations occurred following cooked navy bean intake in dogs, indicating that bean powders may be a viable protein and fiber source for commercial pet foods. The highly variable microbial populations observed in these healthy adult pet dogs at baseline is one potential reason for the difficulty to detect alterations in microbial populations following dietary changes. Given the potential physiological benefits of bean intake in humans and dogs, further evaluation of the impacts of cooked navy bean intake on fecal microbial populations with higher power or more sensitive methods are warranted.
煮熟的豆粉是一种有前途的新型犬类蛋白质和膳食纤维来源，它具有改变微生物组成和功能以控制慢性疾病的潜力。本研究旨在确定作为主食成分的煮熟海军豆粉对健康成年宠物犬粪便微生物群的影响。
方法/主要发现：在进行饮食控制之前和用宏量和微量营养素匹配的饮食进行 4 周饮食治疗后，分别从健康犬的粪便中采集样本，这些饮食分别含有 0%或 25%煮熟的海军豆（n=11 和 n=10），通过 16S rRNA 基因的 454 焦磷酸测序进行分析。经过 4 周的治疗后，喂食对照饮食和海军豆饮食的犬之间差异较小。这些数据表明，海军豆的加入对微生物种群没有重大影响。然而，在两种研究饮食上都观察到由于饮食干预而产生的显著差异（即，基线时的微生物种群与 0%或 25%海军豆饮食干预 4 周后的微生物种群）。在接受对照或海军豆饮食干预 4 周后，与基线相比，厚壁菌门增加，放线菌门和梭杆菌门减少。
犬摄入煮熟的海军豆后，微生物种群没有发生负面变化，这表明豆粉可能是商业宠物食品中一种可行的蛋白质和膳食纤维来源。这些健康成年宠物犬基线时高度可变的微生物种群是在饮食变化后难以检测到微生物种群变化的一个潜在原因。鉴于豆类摄入对人类和犬类的潜在生理益处，需要用更高的功率或更敏感的方法进一步评估煮熟的海军豆摄入对粪便微生物群的影响。
