Khabbaz R F, Hartley T M, Lairmore M D, Kaplan J E
Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, GA 30333.
Am J Public Health. 1990 Feb;80(2):190-2. doi: 10.2105/ajph.80.2.190.
We tested 196 sera from a human T lymphotropic virus type I (HTLV-I) risk group (prostitute women) with two commercial "research" enzyme-linked immunoabsorbent assays (EIA) for HTLV-I antibodies. All tested sera were characterized by HTLV-I Western immunoblots and by HTLV-I radioimmunoprecipitation assays. The estimated sensitivities of the EIA tests were 93.8 percent and 100 percent, and the specificities were 98.8 percent and 95.8 percent, respectively, using recommended criteria for seropositivity (requiring reactivity to both gag p24 and env gp46 or gp61/68). Calculated negative predictive values remained excellent (greater than 99.9 percent and 100 percent, respectively) at lower seroprevalence rates but the positive predictive values were only 7.3 percent and 2.3 percent when calculated for a seroprevalence rate of 0.1 percent. These results emphasize the importance and need for additional HTLV-I supplementary serologic testing when screening populations with low HTLV-I seroprevalence rates.
我们用两种商业化的“研究用”酶联免疫吸附测定法（EIA）检测了196份来自人类嗜T淋巴细胞病毒I型（HTLV-I）风险群体（妓女）的血清，以检测HTLV-I抗体。所有检测血清均通过HTLV-I免疫印迹法和HTLV-I放射免疫沉淀法进行鉴定。使用血清阳性的推荐标准（要求对gag p24和env gp46或gp61/68均有反应性），EIA检测的估计敏感性分别为93.8%和100%，特异性分别为98.8%和95.8%。在较低的血清阳性率下，计算出的阴性预测值仍然极佳（分别大于99.9%和100%），但当血清阳性率为0.1%时计算的阳性预测值仅为7.3%和2.3%。这些结果强调了在筛查HTLV-I血清阳性率较低的人群时，进行额外的HTLV-I补充血清学检测的重要性和必要性。
