University of Copenhagen, Department of Geosciences and Natural Resource Management, Rolighedsvej 23, DK-1958 Frederiksberg C, Denmark.
Bioresour Technol. 2013 Nov;148:180-8. doi: 10.1016/j.biortech.2013.08.130. Epub 2013 Aug 31.
Different versions of two commercial cellulases were tested for their recyclability of enzymatic activity at high dry matter processes (12% or 25% DM). Recyclability was assessed by measuring remaining enzyme activity in fermentation broth and the ability of enzymes to hydrolyse fresh, pretreated wheat straw. Industrial conditions were used to study the impact of hydrolysis temperature (40 or 50°C) and residence time on recyclability. Enzyme recycling at 12% DM indicated that hydrolysis at 50°C, though ideal for ethanol yield, should be kept short or carried out at lower temperature to preserve enzymatic activity. Best results for enzyme recycling at 25% DM was 59% and 41% of original enzyme load for a Celluclast:Novozyme188 mixture and a modern cellulase preparation, respectively. However, issues with stability of enzymes and their strong adsorption to residual solids still pose a challenge for applicable methods in enzyme recycling.
研究了两种商业化纤维素酶在高干物质（12%或 25%DM）过程中的酶活性可回收性。通过测量发酵液中剩余酶活性以及酶对新鲜预处理的小麦秸秆的水解能力来评估可回收性。采用工业条件研究了水解温度（40 或 50°C）和停留时间对可回收性的影响。在 12%DM 下进行酶回收时，结果表明，尽管 50°C 的水解有利于乙醇产量，但为了保持酶活性，应保持较短的水解时间或在较低温度下进行水解。在 25%DM 下进行酶回收的最佳结果分别为原始酶负荷的 59%和 41%，对于 Celluclast:Novozyme188 混合物和现代纤维素酶制剂。然而，酶的稳定性及其对残余固体的强烈吸附仍然是酶回收中适用方法的挑战。
