Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Bioresour Technol. 2011 Sep;102(18):8628-34. doi: 10.1016/j.biortech.2011.03.053. Epub 2011 Mar 22.
A newly isolated indigenous bacterium Pseudomonas sp. CL3 was able to produce novel cellulases consisting of endo-β-1,4-d-glucanase (80 and 100 kDa), exo-β-1,4-d-glucanase (55 kDa) and β-1,4-d-glucosidase (65 kDa) characterized by enzyme assay and zymography analysis. In addition, the CL3 strain also produced xylanase with a molecular weight of 20 kDa. The optimal temperature for enzyme activity was 50, 45, 45 and 55 °C for endo-β-1,4-d-glucanase, exo-β-1,4-d-glucanase, β-1,4-d-glucosidase and xylanase, respectively. All the enzymes displayed optimal activity at pH 6.0. The cellulases/xylanase could hydrolyze cellulosic materials very effectively and were thus used to hydrolyze natural agricultural waste (i.e., bagasse) for clean energy (H2) production by Clostridium pasteurianum CH4 using separate hydrolysis and fermentation process. The maximum hydrogen production rate and cumulative hydrogen production were 35 ml/L/h and 1420 ml/L, respectively, with a hydrogen yield of around 0.96 mol H2/mol glucose.
新分离的土著菌 Pseudomonas sp. CL3 能够产生新型纤维素酶，包括内切-β-1,4-葡聚糖酶（80 和 100 kDa）、外切-β-1,4-葡聚糖酶（55 kDa）和β-1,4-葡萄糖苷酶（65 kDa），通过酶活性测定和同工酶分析进行鉴定。此外，CL3 菌株还产生了分子量为 20 kDa 的木聚糖酶。内切-β-1,4-葡聚糖酶、外切-β-1,4-葡聚糖酶、β-1,4-葡萄糖苷酶和木聚糖酶的最适酶活性温度分别为 50、45、45 和 55°C。所有酶在 pH 6.0 时显示出最佳活性。这些纤维素酶/木聚糖酶可以有效地水解纤维素材料，因此被用于水解天然农业废弃物（如甘蔗渣），以便利用巴氏梭菌 CH4 通过单独水解和发酵工艺生产清洁能源（H2）。最大产氢率和累积产氢量分别为 35 ml/L/h 和 1420 ml/L，氢气产率约为 0.96 mol H2/mol 葡萄糖。
