Tsukahara Tamotsu, Haniu Hisao
Department of Integrative Physiology and Bio-System Control, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
ISRN Inflamm. 2012 Nov 25;2012:983420. doi: 10.5402/2012/983420. eCollection 2012.
Chemokines are regulatory proteins that play an important role in muscle cell migration and proliferation. In this study, C2C12 cells treated with lysophosphatidic acid (LPA) showed an increase in endogenous monocyte chemotactic protein-1 (MCP-1) expression and secretion. LPA is a naturally occurring bioactive lysophospholipid with hormone- and growth-factor-like activities. LPA is produced by activated platelets, cytokine-stimulated leukocytes, and possibly by other cell types. However, the LPA analog cyclic phosphatidic acid (cPA) had no effect on the expression and secretion of MCP-1. LPA, although similar in structure to cPA, had potent inducing effects on MCP-1 expression in C2C12 cells. In this study, we showed that LPA enhanced MCP-1 mRNA expression and protein secretion in a dose-dependent manner. Taken together, these results suggest that LPA enhances MCP-1 secretion in C2C12 cells and thus may play an important role in cell proliferation.
趋化因子是在肌肉细胞迁移和增殖中起重要作用的调节蛋白。在本研究中，用溶血磷脂酸（LPA）处理的C2C12细胞显示内源性单核细胞趋化蛋白-1（MCP-1）的表达和分泌增加。LPA是一种天然存在的具有激素和生长因子样活性的生物活性溶血磷脂。LPA由活化的血小板、细胞因子刺激的白细胞产生，也可能由其他细胞类型产生。然而，LPA类似物环磷酸（cPA）对MCP-1的表达和分泌没有影响。LPA虽然在结构上与cPA相似，但对C2C12细胞中MCP-1的表达有强大的诱导作用。在本研究中，我们表明LPA以剂量依赖的方式增强MCP-1 mRNA表达和蛋白质分泌。综上所述，这些结果表明LPA增强C2C12细胞中MCP-1的分泌，因此可能在细胞增殖中起重要作用。
