Pimentel Gustavo Duarte, Lira Fábio Santos, Rosa José César, Oller do Nascimento Cláudia Maria, Oyama Lila Missae, Harumi Watanabe Regina Lúcia, Ribeiro Eliane Beraldi
Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo (UNIFESP), 04023-062 São Paulo, SP, Brazil.
ISRN Inflamm. 2013 Feb 28;2013:419823. doi: 10.1155/2013/419823. eCollection 2013.
Whether PUFA diets affect inflammatory mediators in central and peripheral sites is not clear. We investigated the effect of high-fat PUFA diets on the expression of proteins involved in inflammatory pathways in hypothalamus, muscle, and liver. Male rats were fed for 2 months with either chow or high-fat diets enriched with either soy (n-6 PUFAs) or fish oil (n-3 PUFAs). The fish group had normal body weight, low serum NEFA, reduced hypothalamic levels of TNF- α , IL-6, and TRAF6, and increased levels of IL-10 receptor. In contrast, the soy group had increased body weight and hypothalamic levels of TRAF6 and NF κ Bp65. In muscle, the fish diet reduced TNF- α and IL-6 levels. Both PUFA diets increased muscle IL-10 levels and reduced liver TNF- α and IL-6 levels. The data showed that the high-fat soy diet induced activation of the hypothalamic NF κ B inflammatory pathway, a feature predisposing to feeding and energy expenditure disturbances associated with the development of obesity. On the other hand, the high-fat fish diet improved the central and the peripheral inflammatory profile via reduction of intracellular inflammatory mediators, suggesting a protection against obesity.
多不饱和脂肪酸（PUFA）饮食是否会影响中枢和外周部位的炎症介质尚不清楚。我们研究了高脂PUFA饮食对下丘脑、肌肉和肝脏中炎症信号通路相关蛋白表达的影响。雄性大鼠分别用普通饲料或富含大豆油（n-6多不饱和脂肪酸）或鱼油（n-3多不饱和脂肪酸）的高脂饲料喂养2个月。鱼油组大鼠体重正常，血清非酯化脂肪酸（NEFA）水平较低，下丘脑肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和肿瘤坏死因子受体相关因子6（TRAF6）水平降低，白细胞介素-10受体水平升高。相比之下，大豆油组大鼠体重增加，下丘脑TRAF6和核因子κB p65（NFκBp65）水平升高。在肌肉中，鱼油饮食降低了TNF-α和IL-6水平。两种PUFA饮食均增加了肌肉中白细胞介素-10（IL-10）水平，并降低了肝脏中TNF-α和IL-6水平。数据表明，高脂大豆油饮食诱导下丘脑NFκB炎症信号通路激活，这一特征易导致与肥胖发生相关的进食和能量消耗紊乱。另一方面，高脂鱼油饮食通过减少细胞内炎症介质改善了中枢和外周炎症状态，提示对肥胖具有保护作用。
