特邀文章：流动红细胞定量相成像中的数字重聚焦
Invited Article: Digital refocusing in quantitative phase imaging for flowing red blood cells.
作者信息
Park Han Sang, Ceballos Silvia, Eldridge Will J, Wax Adam
机构信息
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
出版信息
APL Photonics. 2018 Nov;3(11). doi: 10.1063/1.5043536. Epub 2018 Oct 2.
Abstract
Quantitative phase imaging (QPI) offers high optical path length sensitivity, probing nanoscale features of live cells, but it is typically limited to imaging just few static cells at a time. To enable utility as a biomedical diagnostic modality, higher throughput is needed. To meet this need, methods for imaging cells in flow using QPI are in development. An important need for this application is to enable accurate quantitative analysis. However, this can be complicated when cells shift focal planes during flow. QPI permits digital refocusing since the complex optical field is measured. Here we analyze QPI images of moving red blood cells with an emphasis on choosing a quantitative criterion for digitally refocusing cell images. Of particular interest is the influence of optical absorption which can skew refocusing algorithms. Examples of refocusing of holographic images of flowing red blood cells using different approaches are presented and analyzed.
摘要
定量相位成像（QPI）具有高光程灵敏度，可探测活细胞的纳米级特征，但通常一次只能对少数静态细胞进行成像。为了作为一种生物医学诊断方式发挥作用，需要更高的通量。为满足这一需求，正在开发使用QPI对流动细胞进行成像的方法。该应用的一个重要需求是能够进行准确的定量分析。然而，当细胞在流动过程中改变焦平面时，这可能会变得复杂。由于测量了复杂光场，QPI允许数字重新聚焦。在这里，我们分析移动红细胞的QPI图像，重点是选择用于数字重新聚焦细胞图像的定量标准。特别令人感兴趣的是光吸收的影响，它可能会使重新聚焦算法产生偏差。展示并分析了使用不同方法对流动红细胞全息图像进行重新聚焦的示例。
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