Gahleitner Florian, Guallar-Hoyas Cristina, Beardsmore Caroline S, Pandya Hitesh C, Thomas Cl Paul
Department of Infection, Immunity & Inflammation (Child Health), University of Leicester, Robert Kilpatrick Clinical Sciences Building, Leicester Royal Infirmary, LE1 9HN, UK.
Bioanalysis. 2013 Sep;5(18):2239-47. doi: 10.4155/bio.13.184.
In-community non-invasive identification of asthma-specific volatile organic compounds (VOCs) in exhaled breath presents opportunities to characterize phenotypes, and monitor disease state and therapies. The feasibility of breath sampling with children and the preliminary identification of childhood asthma markers were studied.
End-tidal exhaled breath was sampled (2.5 dm³) from 11 children with asthma and 12 healthy children with an adaptive breath sampler. VOCs were collected onto a Tenax®/Carbotrap hydrophobic adsorbent trap, and analyzed by GC-MS. Classification was by retention-index and mass spectra in a 'breath matrix' followed by multivariate analysis.
A panel of eight candidate markers (1-(methylsulfanyl)propane, ethylbenzene, 1,4-dichlorobenzene, 4-isopropenyl-1-methylcyclohexene, 2-octenal, octadecyne, 1-isopropyl-3-methylbenzene and 1,7-dimethylnaphtalene) were found to differentiate between the asthmatic and healthy children in the test cohort with complete separation by 2D principal components analysis (2D PCA). Furthermore, the breath sampling protocol was found to be acceptable to children and young people.
This method was found to be acceptable for children, and healthy and asthmatic individuals were distinguished on the basis of eight VOCs at elevated levels in the breath of asthmatic children.
在社区中对呼出气中哮喘特异性挥发性有机化合物（VOCs）进行非侵入性鉴定，为表征疾病表型、监测疾病状态和治疗提供了机会。本研究探讨了对儿童进行呼出气采样的可行性以及儿童哮喘标志物的初步鉴定。
使用适应性呼出气采样器，从11名哮喘儿童和12名健康儿童中采集终末潮气呼出气（2.5立方分米）。VOCs被收集到Tenax®/Carbotrap疏水吸附阱上，并通过气相色谱-质谱联用仪进行分析。通过保留指数和“呼吸基质”中的质谱进行分类，随后进行多变量分析。
发现一组八个候选标志物（1-（甲硫基）丙烷、乙苯、1,4-二氯苯、4-异丙烯基-1-甲基环己烯、2-辛烯醛、十八炔、1-异丙基-3-甲基苯和1,7-二甲基萘）能够区分测试队列中的哮喘儿童和健康儿童，通过二维主成分分析（2D PCA）实现了完全分离。此外，发现该呼出气采样方案为儿童和青少年所接受。
该方法被发现对儿童是可接受的，并且基于哮喘儿童呼出气中八种水平升高的VOCs区分了健康个体和哮喘个体。
