Weller B I, Simmons P D, Ivanyi L
Department of Prosthetic Dentistry, Homerton Hospital, London, United Kingdom.
Clin Immunol Immunopathol. 1990 Mar;54(3):347-53. doi: 10.1016/0090-1229(90)90048-u.
The aims of this study were to identify the immunodominant protein antigens of Candida albicans in patients with superficial infections of the oral and vaginal mucosa. Cytoplasmic protein extract from C. albicans was analyzed by the immunoblot technique using sera from 20 patients with chronic atrophic oral candidosis, from 8 patients with vulvovaginal candidosis, and from 20 control subjects. A significantly greater proportion of patient sera reacted with the 65- and 32-kDa antigens when compared with sera from controls (P less than 0.001). Sera from patients also reacted more often with 38- and 29-kDa antigens (P less than 0.05), while sera from both patients and controls reacted with the 47-kDa antigen. The identified 65-, 38-, 32-, and 29-kDa antigens may be of importance for the development and also for the recurrence of superficial candidosis of the oral and/or vaginal mucosa.
本研究的目的是确定口腔和阴道黏膜浅表感染患者白色念珠菌的免疫显性蛋白抗原。采用免疫印迹技术，使用来自20例慢性萎缩性口腔念珠菌病患者、8例外阴阴道念珠菌病患者以及20例对照者的血清，对白色念珠菌的细胞质蛋白提取物进行分析。与对照者血清相比，患者血清中与65 kDa和32 kDa抗原发生反应的比例显著更高（P<0.001）。患者血清与38 kDa和29 kDa抗原发生反应的频率也更高（P<0.05），而患者和对照者的血清均与47 kDa抗原发生反应。所鉴定出的65 kDa、38 kDa、32 kDa和29 kDa抗原可能对口腔和/或阴道黏膜浅表念珠菌病的发生及复发具有重要意义。
