Ivanyi L, Ivanyi J
Department of Clinical Pathology and Immunology, Hammersmith Hospital, London, United Kingdom.
J Infect Dis. 1990 Aug;162(2):519-22. doi: 10.1093/infdis/162.2.519.
Antibody levels to the mycobacterial 65-kDa stress protein (mSP65) were determined by ELISA in sera from patients with chronic atrophic oral candidiasis, vulvovaginal candidiasis, Candida albicans-infected or noninfected oral lichen planus, or recurrent aphthous ulceration and from subjects with clinically healthy oral mucosa. The results showed significantly elevated anti-mSP65 antibody levels in patients with oral or vulvovaginal candidiasis and in patients with Candida-infected lesions of lichen planus when compared with patients with noninfected lichen planus or recurrent oral ulceration and with matched healthy controls (P less than .001). Immunoblot analysis showed that the rabbit antiserum that strongly bound to mSP65 cross-reacted only weakly with a homologous band of a soluble C. albicans extract. Moreover, the binding of antibodies from patients with candidiasis to the mSP65 antigen was not inhibited in the presence of Candida extract. In view of the poor serologic cross-reactivity, it seems plausible that the recall stimulation of anti-mSP65-producing B cells could be induced by helper T cells that cross-react with the structurally homologous protein of C. albicans.
采用酶联免疫吸附测定法（ELISA）检测慢性萎缩性口腔念珠菌病、外阴阴道念珠菌病、白色念珠菌感染或未感染的口腔扁平苔藓、复发性阿弗他溃疡患者以及口腔黏膜临床健康受试者血清中抗分枝杆菌65-kDa应激蛋白（mSP65）的抗体水平。结果显示，与未感染的扁平苔藓患者、复发性口腔溃疡患者以及匹配的健康对照相比，口腔或外阴阴道念珠菌病患者以及念珠菌感染的扁平苔藓患者血清中的抗mSP65抗体水平显著升高（P<0.001）。免疫印迹分析表明，与mSP65强烈结合的兔抗血清仅与白色念珠菌可溶性提取物的同源条带发生微弱交叉反应。此外，在存在念珠菌提取物的情况下，念珠菌病患者抗体与mSP65抗原的结合并未受到抑制。鉴于血清学交叉反应较弱，似乎有可能是与白色念珠菌结构同源蛋白发生交叉反应的辅助性T细胞诱导了产生抗mSP65的B细胞的回忆性刺激。
