Stevanović Magdalena, Savanović Igor, Uskoković Vuk, Skapin Srečo D, Bračko Ines, Jovanović Uroš, Uskoković Dragan
Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Knez Mihailova 35/IV, Belgrade 11000, Serbia.
Colloid Polym Sci. 2012 Feb 1;290(3):221-231. doi: 10.1007/s00396-011-2540-7.
A simple and green chemical method has been developed to synthesize stable bare and capped silver nanoparticles based on the reduction of silver ions by glucose and capping by poly(α,γ,L-glutamic acid) (PGA). The use of ammonia during synthesis was avoided. PGA has had a dual role in the synthesis and was used as a capping agent to make the silver nanoparticle more biocompatible and to protect the nanoparticles from agglomerating in the liquid medium. The synthesized PGA-capped silver nanoparticles in the size range 5-45 nm were stable over long periods of time, without signs of precipitation. Morphological examination has shown that the silver nanoparticles had a nearly spherical, multiply twinned structure. The effects of the reaction temperature and the reaction time during the synthesis were investigated too. The biocompatibility of the PGA-capped silver nano-particles is discussed in terms of in vitro toxicity with human intestinal Caco-2 cells. The samples were characterized by UV-Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurements.
基于葡萄糖还原银离子并通过聚（α，γ，L-谷氨酸）（PGA）进行封端，已开发出一种简单且绿色的化学方法来合成稳定的裸银纳米颗粒和封端银纳米颗粒。合成过程中避免使用氨。PGA在合成中具有双重作用，用作封端剂以使银纳米颗粒更具生物相容性，并保护纳米颗粒在液体介质中不发生团聚。合成的尺寸范围为5 - 45 nm的PGA封端银纳米颗粒在很长一段时间内都是稳定的，没有沉淀迹象。形态学检查表明，银纳米颗粒具有近乎球形的多重孪晶结构。还研究了合成过程中反应温度和反应时间的影响。从与人类肠道Caco - 2细胞的体外毒性方面讨论了PGA封端银纳米颗粒的生物相容性。通过紫外可见光谱、场发射扫描电子显微镜、透射电子显微镜和zeta电位测量对样品进行了表征。
