Grant M D, Weaver M S, Tsoukas C, Hoffmann G W
Department of Microbiology, University of British Columbia, Vancouver, Canada.
J Immunol. 1990 Feb 15;144(4):1241-50.
Autoimmunity often precedes the onset of AIDS-related complex or AIDS, and a number of autoantibodies have been described in AIDS patients and persons at risk for AIDS. The presence of such antibodies provokes speculation that autoimmunity is a component of AIDS pathogenesis. We report evidence of an autoantibody (anticollagen) common to all homosexual AIDS patients studied. High titer serum reactivity against collagen was detected in all homosexual AIDS patients, and in HIV+ homosexuals (66%), HIV+ i.v. drug users (38%) HIV- homosexuals (32%), HIV+ transfusion recipients (22%), and HIV+ hemophiliacs (13%), but not in HIV- i.v. drug users, HIV- transfusion recipients, HIV- hemophiliacs, rheumatoid arthritis patients, or controls. Anticollagen reactivity does not correlate with serum IgG levels, so it is not merely a reflection of polyclonal B-cell activation. Titration of anticollagen positive sera typically revealed anticollagen antibody titers 100 times those of normal sera. Affinity purification and immunoblot analysis confirmed the antibody nature of the anticollagen reactivity. The anticollagen antibodies react preferentially with primary determinants of types I and III collagen revealed after heat denaturation. Similar antibodies occur infrequently in rheumatoid arthritis patients, more often on SLE, and frequently in graft vs host disease and lepromatous leprosy. Levels of anticollagen activity in HIV+ i.v. drug users and transfusion recipients correlate with serum beta 2-microglobulin levels, suggesting that those persons with anticollagen antibodies are at greater risk of developing AIDS. This correlation, the fact that anticollagen antibodies occurred in all homosexual AIDS patients tested, and the occurrence of antibodies against denatured collagen in immune disorders with features similar to AIDS suggest these antibodies may be related to disease progression. The association of anticollagen autoantibodies with AIDS and certain other infections and immune disorders may reflect common immunopathogenic features in the etiology of these disorders.
自身免疫常常先于艾滋病相关综合征或艾滋病的发病，并且在艾滋病患者及有感染艾滋病风险的人群中已发现多种自身抗体。此类抗体的存在引发了一种推测，即自身免疫是艾滋病发病机制的一个组成部分。我们报告了在所研究的所有同性恋艾滋病患者中均存在一种自身抗体（抗胶原蛋白抗体）的证据。在所有同性恋艾滋病患者中均检测到针对胶原蛋白的高滴度血清反应性，在HIV阳性同性恋者（66%）、HIV阳性静脉吸毒者（38%）、HIV阴性同性恋者（32%）、HIV阳性输血接受者（22%）以及HIV阳性血友病患者（13%）中也检测到了该反应性，但在HIV阴性静脉吸毒者、HIV阴性输血接受者、HIV阴性血友病患者、类风湿关节炎患者或对照组中未检测到。抗胶原蛋白反应性与血清IgG水平无关，因此它不仅仅是多克隆B细胞激活的一种反映。抗胶原蛋白阳性血清的滴定通常显示抗胶原蛋白抗体滴度是正常血清的100倍。亲和纯化和免疫印迹分析证实了抗胶原蛋白反应性的抗体性质。抗胶原蛋白抗体优先与热变性后暴露的I型和III型胶原蛋白的主要决定簇发生反应。类似的抗体在类风湿关节炎患者中很少出现，在系统性红斑狼疮患者中出现得更频繁，在移植物抗宿主病和瘤型麻风患者中则经常出现。HIV阳性静脉吸毒者和输血接受者中的抗胶原蛋白活性水平与血清β2-微球蛋白水平相关，这表明那些具有抗胶原蛋白抗体的人患艾滋病的风险更高。这种相关性、在所有检测的同性恋艾滋病患者中均出现抗胶原蛋白抗体这一事实，以及在具有与艾滋病相似特征的免疫紊乱中出现针对变性胶原蛋白的抗体，表明这些抗体可能与疾病进展有关。抗胶原蛋白自身抗体与艾滋病以及某些其他感染和免疫紊乱的关联可能反映了这些疾病病因中常见的免疫致病特征。
