利用超高场 (17)O 磁共振波谱技术无创测量小鼠脑内的氧代谢。
Non-invasive measurement of cerebral oxygen metabolism in the mouse brain by ultra-high field (17)O MR spectroscopy.
机构信息
Center for MR Research, Department of Radiology, Medical School, University of Minnesota, Minneapolis, Minnesota, USA.
出版信息
J Cereb Blood Flow Metab. 2013 Dec;33(12):1846-9. doi: 10.1038/jcbfm.2013.172. Epub 2013 Sep 25.
Abstract
To assess cerebral energetics in transgenic mouse models of neurologic disease, a robust, efficient, and practical method for quantification of cerebral oxygen consumption is needed. (17)O magnetic resonance spectroscopy (MRS) has been validated to measure cerebral metabolic rate of oxygen (CMRO2) in the rat brain; however, mice present unique challenges because of their small size. We show that CMRO2 measurements with (17)O MRS in the mouse brain are highly reproducible using 16.4 Tesla and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics.
摘要
为了评估神经疾病转基因小鼠模型的大脑能量代谢，需要一种稳健、高效且实用的方法来定量测量大脑耗氧量。（17）O 磁共振波谱（MRS）已被验证可用于测量大鼠大脑中的氧代谢率（CMRO2）；然而，由于小鼠体积小，它们带来了独特的挑战。我们证明，使用 16.4 特斯拉和新设计的氧输送系统，（17）O MRS 可在小鼠大脑中进行高度可重复的 CMRO2 测量。该方法可用于快速且重复地测量小鼠的线粒体功能，无需进行口腔插管，并且具有许多潜在的应用来研究大脑能量代谢。
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