Department of Cellular and Molecular Medicine, Nordea Center for Healthy Aging, Copenhagen N, Denmark.
EMBO J. 2013 Oct 16;32(20):2661-71. doi: 10.1038/emboj.2013.211. Epub 2013 Sep 24.
The eukaryotic cell cycle is conventionally viewed as comprising several discrete steps, each of which must be completed before the next one is initiated. However, emerging evidence suggests that incompletely replicated, or unresolved, chromosomes from S-phase can persist into mitosis, where they present a potential threat to the faithful segregation of sister chromatids. In this review, we provide an overview of the different classes of loci where this 'unfinished S-phase business' can lead to a variety of cytogenetically distinct DNA structures throughout the various steps of mitosis. Furthermore, we discuss the potential ways in which cells might not only tolerate this inevitable aspect of chromosome biology, but also exploit it to assist in the maintenance of genome stability.
真核细胞周期通常被认为包括几个离散的步骤，每个步骤必须在启动下一个步骤之前完成。然而，新出现的证据表明，来自 S 期的不完全复制或未解决的染色体可以持续到有丝分裂期，在那里它们对姐妹染色单体的忠实分离构成潜在威胁。在这篇综述中，我们概述了不同类别的基因座，在这些基因座中，“未完成的 S 期事务”可以导致有丝分裂的各个步骤中出现各种不同的细胞遗传学上不同的 DNA 结构。此外，我们还讨论了细胞不仅可以容忍这种不可避免的染色体生物学方面，还可以利用它来帮助维持基因组稳定性的潜在方法。
