Tabaczar Sabina, Pieniążek Anna, Czepas Jan, Piasecka-Zelga Joanna, Gwoździński Krzysztof, Koceva-Chyła Aneta
Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland.
Gen Physiol Biophys. 2013 Dec;32(4):535-43. doi: 10.4149/gpb_2013048. Epub 2013 Sep 26.
The development of side-effects during doxorubicin-docetaxel (DOX-DTX) chemotherapy is considered as related to generation of oxidative stress by DOX. The addition of docetaxel potentiates this effect. Thus, antioxidants are assumed as a promising remedy for neutralizing deteriorating effects of reactive oxygen species (ROS) in pathological conditions and polyphenolic antioxidants are suitable candidates for such a therapeutic approach. We evaluated the ability of quercetin to attenuate oxidative stress developed during the process of DMBA carcinogenesis and DOX-DTX chemotherapy in the blood plasma of rats bearing mammary tumors. We have found that quercetin significantly improved the plasma nonenzymatic antioxidant capacity (NEAC) and reduced lipid peroxidation, which suggest the beneficial effect of flavonoid. The inclusion of quercetin to the DOX-DTX chemotherapy was also advantageous. A considerable decrease of carbonyls and lipid peroxidation products (TBARS) and improvement of the endogenous antioxidant defense system (an increase of NEAC, thiols and SOD activity) were observed compared to rats treated with DOX-DTX chemotherapy. These results suggest that quercetin could protect blood plasma constituents against oxidative damage evoked by DOX and DTX.
多柔比星 - 多西他赛（DOX - DTX）化疗期间副作用的发生被认为与多柔比星产生的氧化应激有关。多西他赛的加入会增强这种效应。因此，抗氧化剂被认为是在病理条件下中和活性氧（ROS）恶化作用的一种有前景的补救措施，而多酚类抗氧化剂是这种治疗方法的合适候选者。我们评估了槲皮素减轻荷乳腺肿瘤大鼠血浆中在二甲基苯并蒽（DMBA）致癌过程和DOX - DTX化疗过程中产生的氧化应激的能力。我们发现槲皮素显著提高了血浆非酶抗氧化能力（NEAC）并减少了脂质过氧化，这表明黄酮类化合物具有有益作用。在DOX - DTX化疗中加入槲皮素也是有利的。与接受DOX - DTX化疗的大鼠相比，观察到羰基和脂质过氧化产物（TBARS）显著减少，内源性抗氧化防御系统得到改善（NEAC、硫醇和超氧化物歧化酶（SOD）活性增加）。这些结果表明，槲皮素可以保护血浆成分免受DOX和DTX引起的氧化损伤。
