Sun Liping, Hu Yanjie, Niyonsaba Aime, Tong Qiaoxia, Lu Li, Li Huiyu, Jie Shenghua
Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
Clin Exp Med. 2014 Nov;14(4):389-95. doi: 10.1007/s10238-013-0259-0. Epub 2013 Sep 26.
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus (SFTSV) with a high fatality rate. But the immunofunction was still unclear. The objective of our study was to assess the immunofunction in SFTS patients. Immunofunction test with flow cytometry which contains CD3+, CD4+ and CD8+ T lymphocytes, B cells and NK cells would be used for detecting serum samples collected from 34 SFTS cases and 20 healthy donors. We found that CD3+ and CD4+ T lymphocytes were significantly diminished in SFTS compared to normal control. In contrast, the percentage of NK cells was elevated. Further analysis revealed that the number of CD3+ and CD4+ T lymphocytes showed that there was a more robust pattern of depression in acute phase and severe SFTS infection compared to the patients in recovery phase and mild SFTS infection. But NK cells were significantly increased in acute phase and severe SFTS. They reverted to the near normal levels in convalescent phase. Additionally, the levels of CD3+ and CD4+ T lymphocytes progressively decreased in death group when compared with the survival group, but the level of B cells was higher. The damages of immune system were obvious, and the immune dysfunction might be partly responsible for disease progression of patients with SFTSV infection.
发热伴血小板减少综合征（SFTS）是由发热伴血小板减少综合征病毒（SFTSV）引起的一种新发传染病，病死率高。但其免疫功能仍不清楚。我们研究的目的是评估SFTS患者的免疫功能。将采用流式细胞术进行免疫功能检测，检测指标包括CD3⁺、CD4⁺和CD8⁺T淋巴细胞、B细胞和NK细胞，对34例SFTS病例和20例健康供者采集的血清样本进行检测。我们发现，与正常对照组相比，SFTS患者的CD3⁺和CD4⁺T淋巴细胞显著减少。相反，NK细胞的百分比升高。进一步分析显示，CD3⁺和CD4⁺T淋巴细胞数量表明，与恢复期患者和轻度SFTS感染患者相比，急性期和重度SFTS感染患者的免疫抑制模式更为明显。但急性期和重度SFTS患者的NK细胞显著增加。它们在恢复期恢复到接近正常水平。此外，与存活组相比，死亡组的CD3⁺和CD4⁺T淋巴细胞水平逐渐降低，但B细胞水平较高。免疫系统受损明显，免疫功能紊乱可能是SFTSV感染患者疾病进展的部分原因。
