Department of Genetics, Development and Cell Biology, Iowa State University, Ames 50011, IA, USA.
Biotechnol Biofuels. 2013 Sep 27;6(1):136. doi: 10.1186/1754-6834-6-136.
Obtaining bioethanol from cellulosic biomass involves numerous steps, among which the enzymatic conversion of the polymer to individual sugar units has been a main focus of the biotechnology industry. Among the cellulases that break down the polymeric cellulose are endoglucanases that act synergistically for subsequent hydrolytic reactions. The endoglucanases that have garnered relatively more attention are those that can withstand high temperatures, i.e., are thermostable. Although our understanding of thermostability in endoglucanases is incomplete, some molecular features that are responsible for increased thermostability have been recently identified. This review focuses on the investigations of endoglucanases and their implications for biofuel applications.
从纤维素生物质中获得生物乙醇涉及多个步骤，其中聚合物转化为单个糖单位的酶促转化一直是生物技术行业的主要关注点。在分解聚合物纤维素的纤维素酶中，协同作用于后续水解反应的内切葡聚糖酶受到了较多关注。相对而言，受到更多关注的内切葡聚糖酶是那些能够耐受高温的，即热稳定的。尽管我们对内切葡聚糖酶的热稳定性的理解并不完整，但最近已经确定了一些导致热稳定性增加的分子特征。本综述重点介绍了内切葡聚糖酶的研究及其在生物燃料应用中的意义。
