Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Anal Chim Acta. 2013 Oct 10;798:33-9. doi: 10.1016/j.aca.2013.08.046. Epub 2013 Aug 31.
A novel aptamer biosensor for cancer cell assay has been reported on the basis of ultrasensitive electrochemical detection. The assay uses the aptamer as a capture probe to recognize and bind the tumor marker on the surface of the cancer cells, forming an aptamer-based sandwich structure for MCF-7 cells detection. Functionalized nanoporous materials, porous graphene oxide/Au composites (GO/Au composites) and porous PtFe alloy have been introduced into the biosensor. Owing to the large surface area and versatile porous structure, the use of nanoporous materials can significantly improve the analysis performance of the biosensors by loading of large amounts of molecules and accelerating diffusion rate. Under the optimized experimental conditions, the proposed aptamer biosensor exhibited excellent analytical performance for MCF-7 cells determination, ranging from 100 to 5.0×10(7) cells mL(-1) with the detection limit of 38 cells mL(-1). The biosensor showed good selectivity, acceptable stability and reproducibility, and developed a highly sensitive and selective method for cancer cells detection.
一种基于超灵敏电化学检测的用于癌细胞分析的新型适体生物传感器已被报道。该分析方法使用适体作为捕获探针来识别和结合癌细胞表面的肿瘤标志物，形成基于适体的 MCF-7 细胞检测三明治结构。功能化纳米多孔材料、多孔氧化石墨烯/金复合材料（GO/Au 复合材料）和多孔 PtFe 合金已被引入生物传感器中。由于具有较大的表面积和多功能的多孔结构，纳米多孔材料的使用可以通过负载大量分子和加速扩散速率来显著提高生物传感器的分析性能。在优化的实验条件下，所提出的适体生物传感器对 MCF-7 细胞的测定表现出优异的分析性能，检测范围为 100 至 5.0×10(7)个细胞 mL(-1)，检测限为 38 个细胞 mL(-1)。该生物传感器表现出良好的选择性、可接受的稳定性和重现性，为癌细胞检测开发了一种高灵敏度和选择性的方法。
