Holland S M, Taylor H R, Gaydos C A, Kappus E W, Quinn T C
Department of Medicine and Dana Center of Preventive Ophthalmology, Wilmer Institute, Johns Hopkins School of Medicine, Baltimore, Maryland 21205.
Infect Immun. 1990 Mar;58(3):593-7. doi: 10.1128/iai.58.3.593-597.1990.
To serially examine the immunopathogenesis and histopathology of infection with Chlamydia pneumoniae, we inoculated two cynomolgus monkeys in the conjunctival sac, nose, and nasopharynx with C. pneumoniae TWAR. After inoculation, C. pneumoniae was isolated from the inoculation sites and the rectums of both monkeys for a period of 5 weeks. After a second inoculation, C. pneumoniae was recovered from the inoculation sites and the rectums of both monkeys for 20 weeks. A third inoculation with C. pneumoniae caused very little productive infection at any site. Prior C. pneumoniae infection did not prevent subsequent C. trachomatis serovar E (Bour strain) infection. Clinical and histopathologic ocular responses to C. pneumoniae infection were mild compared with those to infection with C. trachomatis serovar E. Rectal infection, demonstrated by culture isolation and immunohistopathology, occurred without direct experimental inoculation. Both immunofluorescent staining of mucosal smears with monoclonal antibodies and tissue culture were able to detect C. pneumoniae infection. Experimental nonhuman primate infection with C. pneumoniae appears to be clinically and histopathologically mild and can occur at extrapulmonary sites.
为了连续研究肺炎衣原体感染的免疫发病机制和组织病理学，我们将肺炎衣原体TWAR株接种到两只食蟹猴的结膜囊、鼻腔和鼻咽部。接种后，在5周的时间里，从两只猴子的接种部位和直肠中分离出肺炎衣原体。再次接种后，在20周的时间里从两只猴子的接种部位和直肠中再次分离出肺炎衣原体。第三次接种肺炎衣原体在任何部位都很少引起有效感染。先前的肺炎衣原体感染并不能预防随后的沙眼衣原体血清型E（布尔菌株）感染。与沙眼衣原体血清型E感染相比，肺炎衣原体感染的临床和组织病理学眼部反应较轻。通过培养分离和免疫组织化学证实，直肠感染在没有直接实验接种的情况下发生。用单克隆抗体对黏膜涂片进行免疫荧光染色和组织培养都能够检测到肺炎衣原体感染。实验性非人灵长类动物肺炎衣原体感染在临床和组织病理学上似乎较轻，并且可以发生在肺外部位。
