State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Colloids Surf B Biointerfaces. 2014 Jan 1;113:134-45. doi: 10.1016/j.colsurfb.2013.08.030. Epub 2013 Sep 4.
To improve the antimicrobial ability and cytocompatibility of biomedical titanium implants, many efforts have been made to modify their surface topography and chemical composition. In this work, Ag plasma-modified hierarchical TiO2 film was fabricated on titanium surface via acid etching to produce micropit, hydrothermal treatment to generate TiO2 nanorod and subsequent plasma immersion ion implantation process to impregnate Ag into TiO2 surface. In view of the potential clinical applications, their antimicrobial activity, bioactivity and cytocompatibility were systematically evaluated. The hierarchical TiO2 film showed enhanced bioactivity and bacteriostatic effect on both microbes due to more negative zeta potential, constructing the first defense line against microbial adhesion by electrostatic repulsion. Addition of embedded Ag remarkably enhanced the antimicrobial efficiency toward both microbes based on Schottky contact without Ag(+) release, establishing the second defense line targeting microbial membrane. Furthermore, the addition of Ag degraded the bioactivity very little and exerted nearly no adverse or even promoted effect on MG63 cell functions, including adhesion, spreading and proliferation. This work illustrates a two-defense-line antimicrobial activity in darkness with both prior electrostatic repulsion to inhibit most microbes adhesion and posterior biocidal action to kill residual ones that luckily infiltrated through the first defense line, and provide proof of concept using both clinically relevant human pathogens. In conclusion, the Ag-embedded hierarchical TiO2 film with excellent antimicrobial activity, bioactivity and cytocompatibility provides a promising candidate for orthopedic and dental implants.
为了提高医用钛植入物的抗菌能力和细胞相容性，人们已经做出了许多努力来改变其表面形貌和化学成分。在这项工作中，通过酸蚀在钛表面上制备了具有 Ag 等离子体改性的分级 TiO2 薄膜，以产生微坑，通过水热处理生成 TiO2 纳米棒，随后通过等离子体浸没离子注入工艺将 Ag 注入 TiO2 表面。鉴于潜在的临床应用，系统地评估了它们的抗菌活性、生物活性和细胞相容性。由于具有更负的 ζ 电位，分层 TiO2 薄膜对微生物具有增强的生物活性和抑菌作用，通过静电排斥构建了抵抗微生物附着的第一道防线。嵌入 Ag 的添加显著提高了对两种微生物的抗菌效率，这是基于肖特基接触而没有 Ag+释放，为靶向微生物膜建立了第二道防线。此外，Ag 的添加对生物活性的降低很小，对 MG63 细胞功能几乎没有不良影响，甚至有促进作用，包括粘附、扩散和增殖。这项工作说明了在黑暗中具有双重防御线的抗菌活性，通过静电排斥抑制大多数微生物的附着，随后通过生物杀灭作用杀死侥幸渗透第一道防线的残留微生物，并用两种临床相关的人类病原体提供了概念验证。总之，具有优异抗菌活性、生物活性和细胞相容性的嵌入式分级 TiO2 薄膜为骨科和牙科植入物提供了有前途的候选材料。
