National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Bioresour Technol. 2013 Nov;148:422-7. doi: 10.1016/j.biortech.2013.09.014. Epub 2013 Sep 12.
An advanced sugar-platform bioprocess for lignocellulosic feedstocks by adding a phenolic-acid (PA: p-coumaric acid and ferulic acid) recovery step to the CaCCO process was designed. For efficient PA extraction, pretreatment was 95°C for 2h, producing a yield of 7.30 g/kg-dry rice straw (65.2% of total ester-linked PAs) with insignificant effects on saccharification. PAs were readily recovered in solution during the repeated washings of solids, and the glucose yield, after 72-h saccharification of the washed solids, was significantly improved from 65.9% to 70.3-72.7%, suggesting the removal of potential enzyme inhibitors. The promotion of xylose yield was insignificant, probably due to 13.1-17.8% loss of xylose residues after washing(s). This new bioprocess, termed the SRB (simultaneous recovery of by-products)-CaCCO process, would effectively produce fermentable sugars and other valuables from feedstocks, strengthening the platform in both economic and environmental terms.
设计了一种先进的糖基平台生物工艺，通过向 CaCCO 工艺添加酚酸（PA：对香豆酸和阿魏酸）回收步骤，用于木质纤维素原料。为了实现高效的 PA 提取，预处理在 95°C 下进行 2 小时，以 7.30 g/kg-干稻草的产率（总酯键连接的 PA 的 65.2%）生产，对糖化没有明显影响。在固体的反复洗涤过程中，PA 很容易溶解在溶液中，并且洗涤后的固体经过 72 小时糖化后，葡萄糖产率从 65.9%显著提高到 70.3-72.7%，表明去除了潜在的酶抑制剂。木糖产率的提高不显著，可能是由于洗涤后（s）有 13.1-17.8%的木糖残基损失。这种新的生物工艺，称为 SRB（副产品同时回收）-CaCCO 工艺，将有效地从原料中生产可发酵糖和其他有价值的物质，从经济和环境方面增强平台。
