Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Appl Environ Microbiol. 2013 Dec;79(23):7476-81. doi: 10.1128/AEM.02261-13. Epub 2013 Sep 27.
Temperate bacteriophage WO is a model system for studying tripartite interactions among viruses, bacteria, and eukaryotes, especially investigations of the genomic stability of obligate intracellular bacteria. Few WO genomes exist because of the difficulty in isolating viral DNA from eukaryotic hosts, and most reports are by-products of Wolbachia sequencing. Only one partial genome of a WO phage has been determined directly from isolated particles. We determine the complete genome sequence of prophage WO (WOSol) in Wolbachia strain wSol, which infects the fig wasp Ceratosolen solmsi (Hymenoptera: Chalcidoidea), by high-efficiency thermal asymmetric interlaced PCR. The genome of WOSol is highly degenerated and disrupted by a large region (14,267 bp) from Wolbachia. Consistent with previous molecular studies of multiple WO genomes, the genome of WOSol appears to have evolved by single nucleotide mutations and recombinations.
温和噬菌体 WO 是研究病毒、细菌和真核生物之间三分相互作用的模式系统，特别是研究专性细胞内细菌的基因组稳定性。由于难以从真核宿主中分离病毒 DNA，因此存在的 WO 基因组很少，而且大多数报告都是沃尔巴克氏体测序的副产品。只有一个 WO 噬菌体的部分基因组是直接从分离的颗粒中确定的。我们通过高效热不对称交错 PCR 从感染榕小蜂（膜翅目：长尾小蜂科）的沃尔巴克氏体菌株 wSol 中确定了原噬菌体 WO（WOSol）的完整基因组序列。WOSol 的基因组高度退化，被沃尔巴克氏体的一个大片段（14267bp）破坏。与之前对多个 WO 基因组的分子研究一致，WOSol 的基因组似乎是通过单个核苷酸突变和重组进化而来的。
