Maduro A P, Mansinho K, Teles F, Silva I, Meyer W, Martins M L, Inácio J
Curr Microbiol. 2014 Feb;68(2):199-203. doi: 10.1007/s00284-013-0452-0.
This study provides a comprehensive picture of the C. neoformans/C. gattii molecular types most often associated with human cryptococcosis in Portugal and assesses the impact of C. gattii in these infections. One hundred and twenty-two clinical isolates, from distinct patients, were identified as C. neoformans and genotyped by URA5-RFLP, with the molecular types VNI (45.5 %) and VNIII (30.9 %) being the most commonly found ones. The molecular types VNII (11.4 %) and VNIV (11.4 %) were less abundant. One patient was found to be infected with a VGII isolate. This patient exhibited unusual clinical symptoms of cryptococcosis, reinforcing the suspicion for the presence of a different genotypic pattern, as determined afterwards. This case was detected in 2007 and is the first report of a potential autochthonous C. gattii infection case in Portugal, as the patient revealed no historical record of travelling outside the country.
本研究全面描绘了葡萄牙最常与人类隐球菌病相关的新型隐球菌/格特隐球菌分子类型，并评估了格特隐球菌在这些感染中的影响。从不同患者中分离出122株临床分离株，鉴定为新型隐球菌，并通过URA5-RFLP进行基因分型，其中分子类型VNI（45.5%）和VNIII（30.9%）最为常见。分子类型VNII（11.4%）和VNIV（11.4%）相对较少。发现一名患者感染了VGII分离株。该患者表现出不寻常的隐球菌病临床症状，进一步加深了对存在不同基因型模式的怀疑，随后得到证实。该病例于2007年被检测到，是葡萄牙首例潜在的本地格特隐球菌感染病例报告，因为该患者没有出国旅行的历史记录。
