Researcher, Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, Republic of Korea.
Graduate student, Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, Republic of Korea.
J Prosthet Dent. 2017 Oct;118(4):524-534. doi: 10.1016/j.prosdent.2016.11.014. Epub 2017 Mar 23.
Acrylic resin materials for interim restoration may adversely affect pulp tissue during the polymerization phase.
The purpose of this in vitro study was to determine the cytotoxic and proinflammatory cytokine production effects induced by interim resin materials in primary cultured human dental pulp cells (hDPCs).
Five interim resin materials were evaluated: 3 types of chemically activated products, 1 light-activated product, and 1 computer-aided design and computer-aided manufacturing (CAD-CAM) product. After obtaining eluates from interim resin materials that either were in the process of polymerizing or were already polymerized, these extracts were cocultured with hDPCs under serially diluted conditions (50%, 25%, 12.5%, 6.25%, and 3.125%) for 24 hours with positive (1% phenol) and negative (distilled water) controls. A cell viability assay with tetrazolium was used to evaluate toxic effects on the cells, and images of both live and dead cells were captured using confocal microscopy. Proinflammatory cytokine levels were measured using cytokine antibody arrays. All experiments were independently repeated 3 times, and data were analyzed using 1-way ANOVA and post hoc Tukey honest significant differences test (α=.05).
Cell viabilities less than 70% were observed from the eluates of the 3 chemically activated products under the 50% conditions. Among the chemically activated products, the adverse effects were significantly greater with eluates derived from the polymerizing phase compared than those that had already polymerized, as shown by confocal microscopy images of live and dead cells. However, the light-activated and CAD-CAM-fabricated products did not adversely affect the hDPCs. Significantly increased levels of proinflammatory cytokines were not detected in 12.5% of extract from polymerizing compared with distilled water control.
The 50% eluates derived from chemically activated interim resin during the polymerizing phase were cytotoxic to hDPCs and may adversely affect pulp tissue. Recommendations such as excess washing are necessary during fabrication.
临时修复用的丙烯酸树脂材料可能会在聚合阶段对牙髓组织产生不利影响。
本体外研究的目的是确定临时树脂材料在原代培养的人牙髓细胞（hDPC）中诱导的细胞毒性和促炎细胞因子产生的影响。
评估了 5 种临时树脂材料：3 种化学激活产品、1 种光激活产品和 1 种计算机辅助设计和计算机辅助制造（CAD-CAM）产品。在获得正在聚合或已经聚合的临时树脂材料的浸提物后，将这些提取物在连续稀释条件下（50%、25%、12.5%、6.25%和 3.125%）与 hDPC 共培养 24 小时，阳性（1%苯酚）和阴性（蒸馏水）对照。使用噻唑蓝测定法评估细胞毒性对细胞的影响，并使用共聚焦显微镜拍摄活细胞和死细胞的图像。使用细胞因子抗体阵列测量促炎细胞因子水平。所有实验均独立重复 3 次，使用单因素方差分析和事后 Tukey 显著差异检验（α=.05）对数据进行分析。
在 50%条件下，来自 3 种化学激活产品的浸提物的细胞活力小于 70%。在化学激活产品中，与已经聚合的相比，来自聚合阶段的浸提物对细胞的不良影响更大，这可以通过活细胞和死细胞的共聚焦显微镜图像看出。然而，光激活和 CAD-CAM 制造的产品对 hDPC 没有不良影响。与蒸馏水对照相比，在聚合阶段从聚合物中提取的 12.5%的提取物中未检测到促炎细胞因子水平显著升高。
聚合阶段化学激活临时树脂的 50%浸提物对 hDPC 具有细胞毒性，并可能对牙髓组织产生不利影响。在制造过程中需要进行多余的清洗等建议。
