• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自小鼠二氢叶酸还原酶基因5'侧翼区域的反义链RNA。

Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene.

作者信息

Farnham P J, Abrams J M, Schimke R T

出版信息

Proc Natl Acad Sci U S A. 1985 Jun;82(12):3978-82. doi: 10.1073/pnas.82.12.3978.

DOI:10.1073/pnas.82.12.3978
PMID:2408272
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397917/
Abstract

We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.

摘要

我们已对小鼠二氢叶酸还原酶（EC 1.5.1.3）编码基因5'侧翼区域的一个转录单元进行了特征分析，该转录单元的方向与二氢叶酸还原酶转录方向相反。这些反义链RNA长度为180 - 240个核苷酸，5'端长度不同。它们在核RNA中含量丰富，且未进行多聚腺苷酸化。我们认为二氢叶酸还原酶基因的启动子区域以双向方式发挥作用，既能产生这些RNA，又能产生二氢叶酸还原酶mRNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/34a0f3829a1b/pnas00352-0039-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/526d91342804/pnas00352-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/a34dbc7b55af/pnas00352-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/096713dd705f/pnas00352-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/f5e92339ac19/pnas00352-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/34a0f3829a1b/pnas00352-0039-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/526d91342804/pnas00352-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/a34dbc7b55af/pnas00352-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/096713dd705f/pnas00352-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/f5e92339ac19/pnas00352-0039-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a9e/397917/34a0f3829a1b/pnas00352-0039-c.jpg

相似文献

1
Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene.来自小鼠二氢叶酸还原酶基因5'侧翼区域的反义链RNA。
Proc Natl Acad Sci U S A. 1985 Jun;82(12):3978-82. doi: 10.1073/pnas.82.12.3978.
2
Murine dihydrofolate reductase transcripts through the cell cycle.小鼠二氢叶酸还原酶转录本在细胞周期中的情况。
Mol Cell Biol. 1986 Feb;6(2):365-71. doi: 10.1128/mcb.6.2.365-371.1986.
3
Identification of a new promoter upstream of the murine dihydrofolate reductase gene.小鼠二氢叶酸还原酶基因上游新启动子的鉴定。
Mol Cell Biol. 1989 Oct;9(10):4568-70. doi: 10.1128/mcb.9.10.4568-4570.1989.
4
Independent 5' and 3'-end determination of multiple dihydrofolate reductase transcripts.多个二氢叶酸还原酶转录本5'端和3'端的独立测定
Mol Cell Biol. 1987 Oct;7(10):3732-9. doi: 10.1128/mcb.7.10.3732-3739.1987.
5
Heterogeneity at the 5' termini of mouse dihydrofolate reductase mRNAs. Evidence for multiple promoter regions.小鼠二氢叶酸还原酶mRNA 5'末端的异质性。多个启动子区域的证据。
J Biol Chem. 1985 Feb 25;260(4):2307-14.
6
In vitro transcription and delimitation of promoter elements of the murine dihydrofolate reductase gene.小鼠二氢叶酸还原酶基因启动子元件的体外转录与界定
Mol Cell Biol. 1986 Jul;6(7):2392-401. doi: 10.1128/mcb.6.7.2392-2401.1986.
7
The bidirectionally transcribed dihydrofolate reductase and rep-3a promoters are growth regulated by distinct mechanisms.双向转录的二氢叶酸还原酶和rep - 3a启动子受不同机制调控生长。
Cell Growth Differ. 1995 May;6(5):541-8.
8
Transcript initiation, polyadenylation, and functional promoter mapping for the dihydrofolate reductase-thymidylate synthase gene of Toxoplasma gondii.刚地弓形虫二氢叶酸还原酶-胸苷酸合成酶基因的转录起始、聚腺苷酸化及功能启动子图谱分析
Mol Biochem Parasitol. 2004 Oct;137(2):229-38. doi: 10.1016/j.molbiopara.2003.12.015.
9
A 165-base pair sequence between the dihydrofolate reductase gene and the divergently transcribed upstream gene is sufficient for bidirectional transcriptional activity.二氢叶酸还原酶基因与反向转录的上游基因之间一段165个碱基对的序列足以实现双向转录活性。
J Biol Chem. 1989 Dec 5;264(34):20171-4.
10
Transcription factor Sp1 recognizes a DNA sequence in the mouse dihydrofolate reductase promoter.转录因子Sp1识别小鼠二氢叶酸还原酶启动子中的一个DNA序列。
Nature. 1986;319(6050):246-8. doi: 10.1038/319246a0.

引用本文的文献

1
Human cells contain natural double-stranded RNAs with potential regulatory functions.人类细胞中含有具有潜在调节功能的天然双链 RNA。
Nat Struct Mol Biol. 2015 Jan;22(1):89-97. doi: 10.1038/nsmb.2934. Epub 2014 Dec 15.
2
A novel transforming growth factor beta2 antisense transcript in mammalian lung.哺乳动物肺中一种新型的转化生长因子β2反义转录物。
Biochem J. 1998 Jun 1;332 ( Pt 2)(Pt 2):297-301. doi: 10.1042/bj3320297.
3
Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene.

本文引用的文献

1
Size heterogeneity in the 3' end of dihydrofolate reductase messenger RNAs in mouse cells.小鼠细胞中二氢叶酸还原酶信使核糖核酸3'端的大小异质性
Cell. 1980 Nov;22(2 Pt 2):361-70. doi: 10.1016/0092-8674(80)90346-3.
2
Unstable amplification of an altered dihydrofolate reductase gene associated with double-minute chromosomes.与双微体染色体相关的改变的二氢叶酸还原酶基因的不稳定扩增。
Cell. 1981 Nov;26(3 Pt 1):355-62. doi: 10.1016/0092-8674(81)90204-x.
3
The TATA homology and the mRNA 5' untranslated sequence are not required for expression of essential adenovirus E1A functions.
PR264/SC35剪接因子基因反义转录产生的多种可变RNA的特征分析
Nucleic Acids Res. 1997 Nov 15;25(22):4513-22. doi: 10.1093/nar/25.22.4513.
4
In vitro and in vivo action of antisense RNA.反义RNA的体外和体内作用。
Mol Biotechnol. 1996 Aug;6(1):7-15. doi: 10.1007/BF02762319.
5
Cloning and characterization of a naturally occurring antisense RNA to human thymidylate synthase mRNA.人胸苷酸合成酶信使核糖核酸天然反义核糖核酸的克隆与特性分析
Nucleic Acids Res. 1993 Apr 25;21(8):1747-52. doi: 10.1093/nar/21.8.1747.
6
Identification of a novel sequence element in the common promoter region of human collagen type IV genes, involved in the regulation of divergent transcription.在人类IV型胶原蛋白基因的共同启动子区域中鉴定出一种新型序列元件，其参与了差异转录的调控。
Biochem J. 1993 Jun 15;292 ( Pt 3)(Pt 3):687-95. doi: 10.1042/bj2920687.
7
Conserved cis-elements bind a protein complex that regulates Drosophila ras2/rop bidirectional expression.保守的顺式元件结合一种调节果蝇ras2/rop双向表达的蛋白质复合物。
Br J Cancer. 1994 Feb;69(2):264-73. doi: 10.1038/bjc.1994.50.
8
The canine betaine gamma-amino-n-butyric acid transporter gene: diverse mRNA isoforms are regulated by hypertonicity and are expressed in a tissue-specific manner.犬甜菜碱γ-氨基丁酸转运体基因：多种mRNA异构体受高渗调节，并以组织特异性方式表达。
Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1072-6. doi: 10.1073/pnas.92.4.1072.
9
In vitro transcription and delimitation of promoter elements of the murine dihydrofolate reductase gene.小鼠二氢叶酸还原酶基因启动子元件的体外转录与界定
Mol Cell Biol. 1986 Jul;6(7):2392-401. doi: 10.1128/mcb.6.7.2392-2401.1986.
10
Murine dihydrofolate reductase transcripts through the cell cycle.小鼠二氢叶酸还原酶转录本在细胞周期中的情况。
Mol Cell Biol. 1986 Feb;6(2):365-71. doi: 10.1128/mcb.6.2.365-371.1986.
基本腺病毒E1A功能的表达不需要TATA同源序列和mRNA 5'非翻译序列。
Cell. 1982 May;29(1):139-48. doi: 10.1016/0092-8674(82)90098-8.
4
Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.缺乏二氢叶酸还原酶活性的中国仓鼠细胞突变体的分离
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4216-20. doi: 10.1073/pnas.77.7.4216.
5
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.通过对体内特定缺失突变体的研究鉴定H2A组蛋白基因前奏序列中的调控序列。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6. doi: 10.1073/pnas.77.3.1432.
6
Transcription in vivo from SV40 early promoter deletion mutants without repression by large T antigen.来自SV40早期启动子缺失突变体的体内转录，不受大T抗原抑制。
J Mol Appl Genet. 1983;2(1):127-35.
7
Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
8
In vitro and in vivo analysis of the control of dihydrofolate reductase gene transcription in serum-stimulated mouse fibroblasts.血清刺激的小鼠成纤维细胞中二氢叶酸还原酶基因转录调控的体外和体内分析
J Cell Physiol. 1984 Jan;118(1):79-86. doi: 10.1002/jcp.1041180114.
9
3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.信使核糖核酸的3'端编辑：序列要求及一段60个核苷酸的核糖核酸在组蛋白信使核糖核酸前体成熟过程中的作用
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1057-61. doi: 10.1073/pnas.81.4.1057.
10
A comprehensive sequence analysis program for the IBM personal computer.一款适用于IBM个人计算机的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581-99. doi: 10.1093/nar/12.1part2.581.