Vinegoni Claudio, Lee Sungon, Gorbatov Rostic, Weissleder Ralph
Intravital. 2012 Oct 1;1(2):115-121. doi: 10.4161/intv.23017.
Motion artifacts continue to present a major challenge to single cell imaging in cardiothoracic organs such as the beating heart, blood vessels, or lung. In this study, we present a new water-immersion suctioning stabilizer that enables minimally invasive intravital fluorescence microscopy using water-based stick objectives. The stabilizer works by reducing major motion excursions and can be used in conjunction with both prospective or retrospective gating approaches. We show that the new approach offers cellular resolution in the beating murine heart without perturbing normal physiology. In addition, because this technique allows multiple areas to be easily probed, it offers the opportunity for wide area coverage at high resolution.
运动伪影仍然是心脏、血管或肺等心胸器官单细胞成像面临的主要挑战。在本研究中，我们提出了一种新型水浸抽吸稳定器，它能够使用水基棒状物镜进行微创活体荧光显微镜检查。该稳定器通过减少主要运动偏移来发挥作用，并且可以与前瞻性或回顾性门控方法结合使用。我们表明，这种新方法在跳动的小鼠心脏中提供了细胞分辨率，同时不会干扰正常生理功能。此外，由于该技术允许轻松探测多个区域，因此它提供了高分辨率大面积覆盖的机会。
