使用X射线自由电子激光的蛋白质串行飞秒晶体学中的辐射损伤
Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser.
作者信息
Lomb Lukas, Barends Thomas R M, Kassemeyer Stephan, Aquila Andrew, Epp Sascha W, Erk Benjamin, Foucar Lutz, Hartmann Robert, Rudek Benedikt, Rolles Daniel, Rudenko Artem, Shoeman Robert L, Andreasson Jakob, Bajt Sasa, Barthelmess Miriam, Barty Anton, Bogan Michael J, Bostedt Christoph, Bozek John D, Caleman Carl, Coffee Ryan, Coppola Nicola, Deponte Daniel P, Doak R Bruce, Ekeberg Tomas, Fleckenstein Holger, Fromme Petra, Gebhardt Maike, Graafsma Heinz, Gumprecht Lars, Hampton Christina Y, Hartmann Andreas, Hauser Günter, Hirsemann Helmut, Holl Peter, Holton James M, Hunter Mark S, Kabsch Wolfgang, Kimmel Nils, Kirian Richard A, Liang Mengning, Maia Filipe R N C, Meinhart Anton, Marchesini Stefano, Martin Andrew V, Nass Karol, Reich Christian, Schulz Joachim, Seibert M Marvin, Sierra Raymond, Soltau Heike, Spence John C H, Steinbrener Jan, Stellato Francesco, Stern Stephan, Timneanu Nicusor, Wang Xiaoyu, Weidenspointner Georg, Weierstall Uwe, White Thomas A, Wunderer Cornelia, Chapman Henry N, Ullrich Joachim, Strüder Lothar, Schlichting Ilme
机构信息
Max-Planck Institut für medizinische Forschung, Jahnstrasse 29, DE-69120 Heidelberg, Germany ; Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, DE-22607 Hamburg, Germany.
出版信息
Phys Rev B Condens Matter Mater Phys. 2011 Dec 1;84(21):214111. doi: 10.1103/PhysRevB.84.214111.
X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.
X射线自由电子激光器可产生高强度飞秒脉冲，有望在样品因辐射损伤而破坏之前获得纳米晶体的高分辨率衍射数据。利用直线加速器相干光源收集的溶菌酶纳米晶体在2keV光子下的衍射强度，通过分子置换进行结构测定，并分析了辐射损伤与脉冲长度和注量的函数关系。对于短至70飞秒的脉冲，观察到了辐射损伤的特征。传统晶体学中使用的参数缩放无法解释所观察到的效应。
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