Cai Shanshan, Song Na, Zhang Liyan, Gao Tianxiang
a Institute of Evolution and Marine Biodiversity, Ocean University of China , Qingdao , China .
b Department of Marine Biology , Fujian Institute Oceanography , Xiamen , People's Republic of China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1499-500. doi: 10.3109/19401736.2014.953110. Epub 2014 Oct 20.
The long PCR and primer walking methods are used for determining the complete mitochondrial genome sequence of Sillago aeolus. The entire sequence is 16,499 bp in length including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 non-coding regions (L-strand replication origin and control region). Within the control region, we identified the termination-associated sequence domain (TAS), central conserved domains (CSB-F, CSB-E, CSB-D, CSB-C, CSB-B and CSB-A), and three conserved sequence blocks (CSB-1, CSB-2 and CSB-3).
采用长PCR和引物步移法测定了多鳞鱚的线粒体全基因组序列。该序列全长16499bp,包括13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和2个非编码区(轻链复制起点和控制区)。在控制区内,我们鉴定出了终止相关序列结构域(TAS)、中央保守结构域(CSB-F、CSB-E、CSB-D、CSB-C、CSB-B和CSB-A)以及三个保守序列块(CSB-1、CSB-2和CSB-3)。