Tsiquaye K N, Rapicetta M, McCaul T F, Zuckerman A J
J Virol Methods. 1985 May;11(1):49-57. doi: 10.1016/0166-0934(85)90124-7.
Inoculation of fertile Pekin duck eggs with diluted serum containing DHBV into eggs incubated for 24 h and into the extra-embryonic cavities of 14-day-old embryos resulted in a high proportion of viraemic ducklings irrespective of the route of inoculation. Long-term observation of som of the ducks established that the viraemia induced experimentally is long-lasting and has persisted for periods up to 16 mth post-hatch. Separation of DHBV from the plasma of carrier ducks by rate zonal centrifugation was examined by DNA polymerase (DNAP) activity. Particles in the fraction with peak DNAP activity had a buoyant density of 1.16 g X cm-3 in sucrose and an estimated sedimentation coefficient, S20.w of 77. DHBV particles, the morphology of which could be resolved under the electron microscope, consisted of a coat (about 10 nm in thickness) surrounding a core with a diameter measuring 40 nm but not 27 nm as previously reported. Spike-like projections were found on the surface of the core as described previously by W.S. Mason, G. Seal and J. Summers, 1980, J. Virol. 36, 829-836.
将含有鸭乙型肝炎病毒（DHBV）的稀释血清接种到已孵化24小时的北京鸭种蛋以及14日龄胚胎的胚外腔中，无论接种途径如何，都会导致高比例的带病毒血症雏鸭。对部分鸭子的长期观察表明，实验诱导的病毒血症持续时间长，在雏鸭孵化后长达16个月仍持续存在。通过DNA聚合酶（DNAP）活性检测，利用速率区带离心法从携带病毒鸭子的血浆中分离DHBV。DNAP活性峰值部分的颗粒在蔗糖中的浮力密度为1.16 g/cm³，估计沉降系数S20,w为77。DHBV颗粒在电子显微镜下可分辨其形态，由一层包膜（厚度约10纳米）围绕着一个直径为40纳米的核心组成，而非如先前报道的27纳米。如W.S. Mason、G. Seal和J. Summers在1980年《病毒学杂志》第36卷第829 - 836页所描述的那样，在核心表面发现了类似穗状的突起。
