Cova L, Hantz O, Arliaud-Gassin M, Chevalier A, Berthillon P, Boulay J, Jacquet C, Chomel B, Vitvitski L, Trepo C
J Virol Methods. 1985 Mar;10(3):251-60. doi: 10.1016/0166-0934(85)90065-5.
Duck hepatitis B virus (DHBV) was found in the serum of 1-6% of Pekin ducklings originated from French commercial flocks. The viremia was followed in the serum of 5 ducklings over a span of 3 mth by monitoring the levels of DHBV DNA and the endogenous DNA polymerase (DNAp) activity. The DHBV DNA levels in serum were quantified either by the DNA dot hybridization technique including counting of retained radioactivity, or by successive dilutions of each serum sample followed by DNA hybridization. The counting of the retained radioactivity was plotted on a curve and its evolution compared with that of viral DNAp activity. DHBV DNA levels in serum, estimated by both methods paralleled those of the DNAp activity, which peaked at the 4th or 5th week posthatch to decrease and fluctuate thereafter. Occasional discordance between DHBV DNA levels and the endogenous DNAp activity was observed, which could be correlated with the degree of repair of the single stranded gap of serum DHBV DNA. Parallel follow up studies comparing quantitative estimations of serum viral DNA and of DNAp activity, as presented here, may provide some clues for the understanding of the mechanisms involved in the establishment of the HEPA DNA virus carrier state. Such comparative studies may also be crucial for optimal monitoring of antiviral drugs in both human clinical trials and animal experimental studies.
在源自法国商业鸭群的1%至6%的北京雏鸭血清中发现了鸭乙型肝炎病毒（DHBV）。通过监测5只雏鸭血清中DHBV DNA水平和内源性DNA聚合酶（DNAp）活性，在3个月的时间内对其病毒血症进行了跟踪研究。血清中DHBV DNA水平通过DNA斑点杂交技术（包括对保留放射性的计数）或通过对每个血清样本进行连续稀释后进行DNA杂交来定量。将保留放射性的计数绘制在曲线上，并将其变化与病毒DNAp活性的变化进行比较。通过两种方法估计的血清中DHBV DNA水平与DNAp活性水平平行，在雏鸭孵化后第4或第5周达到峰值，此后下降并波动。观察到DHBV DNA水平与内源性DNAp活性之间偶尔存在不一致，这可能与血清DHBV DNA单链缺口的修复程度相关。如本文所述，比较血清病毒DNA定量估计和DNAp活性的平行随访研究，可能为理解嗜肝DNA病毒携带者状态建立所涉及的机制提供一些线索。此类比较研究对于人类临床试验和动物实验研究中抗病毒药物的最佳监测也可能至关重要。
