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北京鸭胚胎自然感染鸭乙型肝炎病毒。

Naturally occurring infection of Pekin duck embryos by duck hepatitis B virus.

作者信息

O'Connell A P, Urban M K, London W T

出版信息

Proc Natl Acad Sci U S A. 1983 Mar;80(6):1703-6. doi: 10.1073/pnas.80.6.1703.

DOI:10.1073/pnas.80.6.1703
PMID:6572934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393671/
Abstract

We tested the hypothesis that duck hepatitis B virus (DHBV) is a naturally occurring congenital infection of Pekin duck embryos. Of 219 embryos, 5-25 days after being laid, sera from 30 were found to be positive for endogenous DNA polymerase activity characteristic of hepatitis B-related viruses. The presence of the duck virus was confirmed by hybridization with cloned DHBV DNA. Viral DNA was also found in the livers of embryos incubated for 12 or 18 days. Electrophoretically different forms of DHBV DNA were identified in liver extracts that were not present in serum. These additional liver forms probably represent viral replication intermediates. These observations suggest that the vertical route is a major pathway of DHBV transmission and that viral replication may be initiated by the 12th day of embryonic life.

摘要

我们验证了以下假说

鸭乙型肝炎病毒（DHBV）是北京鸭胚胎自然发生的先天性感染源。在219枚产后5至25天的胚胎中，发现30枚胚胎的血清中内源性DNA聚合酶活性呈阳性，这是乙型肝炎相关病毒的特征。通过与克隆的DHBV DNA杂交，证实了鸭病毒的存在。在孵化12天或18天的胚胎肝脏中也发现了病毒DNA。在肝脏提取物中鉴定出电泳形式不同的DHBV DNA，而血清中不存在这些形式。这些额外的肝脏形式可能代表病毒复制中间体。这些观察结果表明，垂直传播途径是DHBV传播的主要途径，并且病毒复制可能在胚胎发育的第12天就已开始。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55fc/393671/41d7a449f64e/pnas00632-0227-c.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55fc/393671/961f4b8d423c/pnas00632-0225-a.jpg
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