Department of Pediatric Neurology, University Hospital Motol, Prague, Czech Republic.
Fluids Barriers CNS. 2013 Oct 4;10(1):30. doi: 10.1186/2045-8118-10-30.
Chemokines and cytokines in cerebrospinal fluid (CSF) and serum have been extensively studied in adults with neuroborreliosis (NB), whereas there are limited data about the pediatric population. In adults, T helper type 1 (Th1) and Th17-related cytokines were observed during acute NB. In children, the Th2 response is thought to moderate the disease course. The aim of this study was to determine the chemokine-cytokine profile in children with acute NB displaying Borrelia-related peripheral facial nerve palsy (PFNP).
Luminex multiple bead technology was used for the detection of twelve cytokines and chemokines in the CSF and serum of three groups: 1) children with Borrelia-related PFNP (BPFNP); 2) children with non-borrelial "idiopathic" PFNP (NIPFNP); and 3) age-related controls.
In BPFNP, cytokines-chemokines related to a non-specific pro-inflammatory activity and specific Th1/Th17 responses were detected in CSF, and elevated IL-7 and IL-10 levels were observed in serum and CSF compared to NIPFNP and to controls. In NIPFNP, CSF findings were similar to controls; however, higher levels of IL-7 and MCP-1 were observed in serum. Higher IL-8, IL-15 and MCP-1 levels were detected in CSF compared to serum in all groups. MCP-1 and IL-8 levels in CSF were strikingly higher in BPFNP compared to the other two groups, while IL-15 levels in CSF showed no difference. In addition, in controls, increased IL-4 level was found in CSF compared to serum.
The chemokine-cytokine profile in the CSF of children with acute NB was similar to previous studies in adults. Our data suggests that higher levels of IL-4, IL-15 and MCP-1 levels in CSF compared to serum in controls might represent a potentially protective cytokine milieu in the CNS compartment.
在神经莱姆病（NB）的成人患者中，脑脊液（CSF）和血清中的趋化因子和细胞因子已得到广泛研究，而儿科患者的数据有限。在成人中，急性 NB 时观察到辅助性 T 细胞 1（Th1）和 Th17 相关细胞因子。在儿童中，认为 Th2 反应可调节疾病进程。本研究旨在确定表现出伯氏疏螺旋体相关周围性面神经麻痹（PFNP）的急性 NB 儿童的趋化因子-细胞因子谱。
使用 Luminex 多重珠技术检测三组儿童的 CSF 和血清中的 12 种细胞因子和趋化因子：1）伯氏疏螺旋体相关 PFNP（BPFNP）患儿；2）非伯氏疏螺旋体“特发性”PFNP（NIPFNP）患儿；3）年龄匹配的对照组。
在 BPFNP 中，CSF 中检测到与非特异性促炎活性和特异性 Th1/Th17 反应相关的细胞因子-趋化因子，与 NIPFNP 和对照组相比，血清和 CSF 中观察到 IL-7 和 IL-10 水平升高。在 NIPFNP 中，CSF 发现与对照组相似；然而，血清中观察到更高水平的 IL-7 和 MCP-1。与血清相比，所有组的 CSF 中均检测到更高水平的 IL-8、IL-15 和 MCP-1。与其他两组相比，BPFNP 的 CSF 中 MCP-1 和 IL-8 水平明显更高，而 CSF 中 IL-15 水平无差异。此外，在对照组中，与血清相比，CSF 中发现 IL-4 水平升高。
急性 NB 患儿 CSF 的趋化因子-细胞因子谱与先前成人研究相似。我们的数据表明，与血清相比，对照组 CSF 中更高水平的 IL-4、IL-15 和 MCP-1 可能代表中枢神经系统中潜在的保护性细胞因子环境。
