Skogman Barbro H, Hellberg Sandra, Ekerfelt Christina, Jenmalm Maria C, Forsberg Pia, Ludvigsson Johnny, Bergström Sven, Ernerudh Jan
Department of Pediatrics, Falun General Hospital, Sweden.
Clin Dev Immunol. 2012;2012:294587. doi: 10.1155/2012/294587. Epub 2011 Dec 5.
Why some individuals develop clinical manifestations in Lyme borreliosis (LB) while others remain asymptomatic is largely unknown. Therefore, we wanted to investigate adaptive and innate immune responsiveness to Borrelia burgdorferi sensu lato in exposed Borrelia-antibody-positive asymptomatic children (n = 20), children with previous clinical LB (n = 24), and controls (n = 20). Blood samples were analyzed for Borrelia-specific interferon (IFN)-γ, interleukin (IL)-4, and IL-17 secretion by ELISPOT and Borrelia-induced IL-1β, IL-6, IL-10, IL-12(p70), and tumor necrosis factor (TNF) secretion by Luminex. We found no significant differences in cytokine secretion between groups, but a tendency towards an increased spontaneous secretion of IL-6 was found among children with previous clinical LB. In conclusion, the adaptive or innate immune responsiveness to Borrelia burgdorferi sensu lato was similar in Borrelia-exposed asymptomatic children and children with previous clinical LB. Thus, the immunological mechanisms of importance for eradicating the spirochete effectively without developing clinical manifestations of LB remain unknown.
为什么有些人在莱姆病（LB）中会出现临床表现，而另一些人却没有症状，这在很大程度上尚不清楚。因此，我们想要研究暴露于伯氏疏螺旋体的无症状且伯氏疏螺旋体抗体呈阳性的儿童（n = 20）、既往有临床LB的儿童（n = 24）和对照组（n = 20）对伯氏疏螺旋体狭义种的适应性和先天性免疫反应。通过ELISPOT分析血样中伯氏疏螺旋体特异性干扰素（IFN）-γ、白细胞介素（IL）-4和IL-17的分泌情况，通过Luminex分析伯氏疏螺旋体诱导的IL-1β、IL-6、IL-10、IL-12（p70）和肿瘤坏死因子（TNF）的分泌情况。我们发现各组之间细胞因子分泌没有显著差异，但在既往有临床LB的儿童中发现IL-6的自发分泌有增加的趋势。总之，暴露于伯氏疏螺旋体的无症状儿童和既往有临床LB的儿童对伯氏疏螺旋体狭义种的适应性或先天性免疫反应相似。因此，在不出现LB临床表现的情况下有效根除螺旋体的重要免疫机制仍然未知。
