Langsley G, Scherf A, Mercereau-Puijalon O, Koenen M, Kahane B, Mattei D, Guillotte M, Sibilli L, Garner I, Müller-Hill B
Nucleic Acids Res. 1985 Jun 11;13(11):4191-202. doi: 10.1093/nar/13.11.4191.
A genomic expression library of P.falciparum has been differentially screened with a number of immune sera. The response of 9 clones to the various sera is presented, together with the DNA sequence encoding the epitopes. All but one clone are extremely A+T rich and unlike the other P.falciparum epitopes described, are not composed of amino acid repeats. One clone, which responds specifically with a protective serum, has been analysed in detail. The epitope is carried on a 160kd antigen which is transcribed from a single gene to give a protein expressed in all of the erythrocytic forms. DNA sequence of this clone reveals it to have more than one open reading frame, only one of which is transcribed in the blood stages. The possible significance of the other open readings frames is discussed.
已利用多种免疫血清对恶性疟原虫的基因组表达文库进行了差异筛选。展示了9个克隆对各种血清的反应，以及编码表位的DNA序列。除一个克隆外，所有克隆都富含A+T，与所描述的其他恶性疟原虫表位不同，它们不是由氨基酸重复序列组成。对一个与保护性血清特异性反应的克隆进行了详细分析。该表位存在于一种160kd的抗原上，该抗原由单个基因转录产生一种在所有红细胞形式中都表达的蛋白质。该克隆的DNA序列显示它有多个开放阅读框，其中只有一个在血液阶段转录。讨论了其他开放阅读框的可能意义。
