Gácser Attila, Tiszlavicz Zoltán, Németh Tibor, Seprényi György, Mándi Yvette
Department of Microbiology, University of Szeged, Kozep Fasor 52., H-6726 Szeged, Hungary.
Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.
Microbes Infect. 2014 Jan;16(1):80-5. doi: 10.1016/j.micinf.2013.09.003. Epub 2013 Oct 2.
In this study we investigated the effects of Candida albicans, Candida krusei, Candida tropicalis and Candida parapsilosis on human beta-defensin 2 (HBD-2) production in Caco-2 intestinal cell line, and the production of alpha-defensins (human neutrophil peptides, HNP 1-3) in peripheral blood. Opportunistic pathogen yeasts can modulate the host immune function by inducing defensins, the natural antimicrobial peptides. Here we show that Candida spp. stimulated HBD-2 expression in and release from Caco-2 cells, with C. albicans inducing the highest levels of HBD-2. Similarly, HNP 1-3 secretion was significantly increased in whole blood after exposure to Candida yeast cells, with C. albicans producing the greatest effect. Our investigations underscore the important role of beta and alpha defensins produced by intestinal epithelial cells locally and neutrophils systemically in the antifungal defense against Candida.
在本研究中，我们调查了白色念珠菌、克柔念珠菌、热带念珠菌和近平滑念珠菌对Caco-2肠细胞系中人类β-防御素2（HBD-2）产生的影响，以及对外周血中α-防御素（人类中性粒细胞肽，HNP 1-3）产生的影响。机会性病原体酵母可通过诱导天然抗菌肽防御素来调节宿主免疫功能。在此我们表明，念珠菌属可刺激Caco-2细胞中HBD-2的表达及释放，其中白色念珠菌诱导的HBD-2水平最高。同样，暴露于念珠菌酵母细胞后，全血中HNP 1-3的分泌显著增加，白色念珠菌产生的影响最大。我们的研究强调了肠道上皮细胞局部产生的β-防御素和中性粒细胞全身产生的α-防御素在抗念珠菌真菌防御中的重要作用。
