Abner Kristo, Aaviksaar Tõnis, Adamberg Kaarel, Vilu Raivo
Tallinn University of Technology, Department of Chemistry, Akadeemia tee 15, 12618 Tallinn, Estonia; Competence Centre of Food and Fermentation Technologies, Akadeemia tee 15a, 12618 Tallinn, Estonia.
Competence Centre of Food and Fermentation Technologies, Akadeemia tee 15a, 12618 Tallinn, Estonia; Tallinn University of Technology, Department of Food Processing, Ehitajate tee 5, 19086 Tallinn, Estonia.
J Theor Biol. 2014 Jan 21;341:78-87. doi: 10.1016/j.jtbi.2013.09.035. Epub 2013 Oct 5.
One of the recognized prokaryotic cell cycle theories is Cooper-Helmstetter (CH) theory which relates start of DNA replication to particular (initiation) cell mass, cell growth and division. Different aspects of this theory have been extensively studied in the past. In the present study CH theory was applied at single cell level. Universal equations were derived for different cell parameters (cell mass and volume, surface area, DNA amount and content) depending on constructivist cell cycle parameters (unit mass, replication and division times, cell age, cell cycle duration) based on selected growth laws of cell mass (linear, exponential). The equations derived can be integrated into single-cell models for the analysis and design of bacterial cells.
公认的原核细胞周期理论之一是库珀-赫尔姆施泰特（CH）理论，该理论将DNA复制的起始与特定的（起始）细胞质量、细胞生长和分裂联系起来。过去已经对该理论的不同方面进行了广泛研究。在本研究中，CH理论被应用于单细胞水平。根据选定的细胞质量生长规律（线性、指数），基于建构主义细胞周期参数（单位质量、复制和分裂时间、细胞年龄、细胞周期持续时间），推导出了不同细胞参数（细胞质量和体积、表面积、DNA量和含量）的通用方程。所推导的方程可整合到单细胞模型中，用于细菌细胞的分析和设计。
