Broekhuyse R M, Tolhuizen E F, Janssen A P, Winkens H J
Curr Eye Res. 1985 May;4(5):613-8. doi: 10.3109/02713688508999993.
S-antigen has been quantitated in bovine and rat retina by electroimmunoassay. The molar ratios S-antigen to rhodops in in photoreceptor cells were close to 1:1. Immunofluorescence studies show that light induces a shift of S-antigen towards the rod outer segments where it concentrates. Assays indicate that S-antigen becomes largely water insoluble but detergent soluble under these conditions. On basis of previous ultrastructural and present results, this has been interpreted as a light induced binding of S-antigen to the rod outer segment membranes. The data support evidence from literature that S-antigen interacts with (rhod) ops in and we conclude that S-antigen plays a major role in the phototransduction process.
已通过电免疫测定法定量测定了牛和大鼠视网膜中的S抗原。光感受器细胞中S抗原与视紫红质的摩尔比接近1:1。免疫荧光研究表明，光照会使S抗原向杆状外段转移并在那里聚集。分析表明，在这些条件下，S抗原在很大程度上变得不溶于水但可溶于去污剂。根据先前的超微结构和目前的结果，这被解释为光诱导S抗原与杆状外段膜结合。这些数据支持了文献中的证据，即S抗原与视（紫）紫红质相互作用，我们得出结论，S抗原在光转导过程中起主要作用。
