Uusitalo H, Lehtosalo J I, Gregerson D S, Uusitalo R, Palkama A
Graefes Arch Clin Exp Ophthalmol. 1985;222(3):118-22. doi: 10.1007/BF02173534.
Using a peroxidase-antiperoxidase immunohistochemical technique, the light and electron microscopical localization of retinal S-antigen was demonstrated in the rat retina, using antisera to bovine and guinea pig S-antigen. Comparable localization of S-antigen was observed with both antisera, although in our hands, the antiserum to bovine S-antigen gave more intense immunostaining. By light microscopy, the most intense immunoreactivity to retinal S-antigen was seen in the outer segment of the photoreceptor layer. Some immunostaining was also demonstrated surrounding the cell bodies of the outer nuclear layer. The inner segment of photoreceptors was virtually devoid of immunoreactivity. Ultrastructurally, immunoreactivity to S-antigen was demonstrated mainly on the inner surfaces of the visual discs of the rods. The plasma membranes of the outer segment of the rods were stained as well. Immunoreactivity surrounding the cell bodies of the outer nuclear layer was ultrastructurally localized on plasma membranes. The inner segment of the photoreceptor layer, other parts of the retina, or any eye structures other than retina showed no immunoreactivity. The relationship of retinal S-antigen to other cell components in the retina and the possible mechanisms of the autoimmune uveal diseases are discussed.
利用过氧化物酶 - 抗过氧化物酶免疫组织化学技术，使用针对牛和豚鼠S抗原的抗血清，在大鼠视网膜中证明了视网膜S抗原的光镜和电镜定位。两种抗血清均观察到S抗原的类似定位，尽管在我们的实验中，针对牛S抗原的抗血清产生的免疫染色更强。通过光镜观察，在光感受器层的外段可见对视网膜S抗原最强的免疫反应性。在外核层细胞体周围也显示出一些免疫染色。光感受器的内段几乎没有免疫反应性。超微结构上，对S抗原的免疫反应性主要在视杆细胞视盘的内表面显示。视杆细胞外段的质膜也被染色。在外核层细胞体周围的免疫反应性在超微结构上定位于质膜。光感受器层的内段、视网膜的其他部分或视网膜以外的任何眼结构均未显示免疫反应性。本文讨论了视网膜S抗原与视网膜中其他细胞成分的关系以及自身免疫性葡萄膜炎疾病的可能机制。
