Institute of Biological Sciences, Faculty of Science, Laboratory of Biomedical Science and Molecular Microbiology, University of Malaya, 50603 Kuala Lumpur, Malaysia.
J Food Prot. 2013 Oct;76(10):1797-800. doi: 10.4315/0362-028X.JFP-13-141.
Vibrio vulnificus is a highly invasive human pathogen that exists naturally in estuarine environment and coastal waters. In this study, we used different PCR assays to detect V. vulnificus in 260 seafood and 80 seawater samples. V. vulnificus was present in about 34 (13%) of the 260 seafood samples and 18 (23%) of the 80 seawater samples. Repetitive extragenic palindromic PCR (REP-PCR) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) were applied to subtype the V. vulnificus isolates. Twenty-five REP profiles and 45 ERIC profiles were observed, and the isolates were categorized into 9 and 10 distinct clusters at the similarity of 80%, by REP-PCR and ERIC-PCR, respectively. ERIC-PCR is more discriminative than REP-PCR in subtyping V. vulnificus, demonstrating high genetic diversity among the isolates.
创伤弧菌是一种存在于港湾环境和沿海水域中的高侵袭性人类病原体。在这项研究中，我们使用不同的 PCR 检测方法对 260 份海鲜和 80 份海水样本中的创伤弧菌进行了检测。260 份海鲜样本中有约 34 份（13%）和 80 份海水样本中有 18 份（23%）检出创伤弧菌。我们采用重复回文外扩增单位 PCR（REP-PCR）和肠细菌重复基因间一致性 PCR（ERIC-PCR）对创伤弧菌分离株进行了亚型分析。通过 REP-PCR 和 ERIC-PCR 观察到 25 种 REP 图谱和 45 种 ERIC 图谱，分离株在相似度为 80%时分为 9 个和 10 个不同的聚类。ERIC-PCR 在创伤弧菌的分型中比 REP-PCR 更具区分性，表明分离株具有较高的遗传多样性。
