Hernández-Robles M F, Natividad-Bonifacio I, Álvarez-Contreras A K, Tercero-Alburo J J, Quiñones-Ramírez E I, Vázquez-Salinas C
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional,Prolongación Carpio y Plan de Ayala S/N, Ciudad de México, Colonia Santo Tomas,CP. 11340, Mexico.
Departamento de Biotecnología, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San Rafael Atlixco 186, Ciudad de México, Colonia Vicentina,C.P. 09340, Mexico.
Int J Microbiol. 2021 Feb 10;2021:8397930. doi: 10.1155/2021/8397930. eCollection 2021.
is a Gram-negative bacterium that is closely related to and causes gastroenteritis in humans due to contaminated fish consumption and seafood. This bacterium was isolated and identified from 238 analyzed samples of sea water, oysters, and fish. Twenty strains were identified as according to amplification of the gene, which is useful as a marker of identification of the species. The production of lipases, proteases, and nucleases was detected; 45% of the strains were able to produce thermonucleases and 40% were capable of producing hydroxamate-type siderophores, and the fragment of the gene was amplified in all of the strains. Seventy-five percent of strains showed cytopathic effect on Chinese hamster ovary (CHO) cells and destruction of the monolayer, and 100% of the strains were adherent on the HEp-2 cell line with an aggregative adherence pattern. The presence of virulence factors in strains obtained from fishery products suggests that another member of the genus could represent a risk to the consumer due to production of different metabolites that allows it to subsist in the host.
是一种革兰氏阴性菌，与……密切相关，因食用受污染的鱼类和海鲜而导致人类患肠胃炎。该细菌是从238份分析的海水、牡蛎和鱼类样本中分离和鉴定出来的。根据作为该物种鉴定标记的……基因的扩增，鉴定出20株……。检测到脂肪酶、蛋白酶和核酸酶的产生；45%的菌株能够产生热核酸酶，40%能够产生异羟肟酸型铁载体，并且在所有……菌株中均扩增出……基因片段。75%的……菌株对中国仓鼠卵巢（CHO）细胞表现出细胞病变效应并破坏单层，100%的菌株以聚集性粘附模式粘附在HEp-2细胞系上。从渔业产品中获得的……菌株中存在毒力因子，这表明……属的另一个成员可能因产生使其能够在宿主体内存活的不同代谢物而对消费者构成风险。
