Polymer Chemistry & Biomaterials Research Group, Department of Organic Chemistry, Ghent University, Krijgslaan 281 S4 Bis, Ghent B-9000, Belgium.
Biomaterials. 2014 Jan;35(1):49-62. doi: 10.1016/j.biomaterials.2013.09.078. Epub 2013 Oct 7.
In the present study, we report on the combined efforts of material chemistry, engineering and biology as a systemic approach for the fabrication of high viability 3D printed macroporous gelatin methacrylamide constructs. First, we propose the use and optimization of VA-086 as a photo-initiator with enhanced biocompatibility compared to the conventional Irgacure 2959. Second, a parametric study on the printing of gelatins was performed in order to characterize and compare construct architectures. Hereby, the influence of the hydrogel building block concentration, the printing temperature, the printing pressure, the printing speed, and the cell density were analyzed in depth. As a result, scaffolds could be designed having a 100% interconnected pore network in the gelatin concentration range of 10-20 w/v%. In the last part, the fabrication of cell-laden scaffolds was studied, whereby the application for tissue engineering was tested by encapsulation of the hepatocarcinoma cell line (HepG2). Printing pressure and needle shape was revealed to impact the overall cell viability. Mechanically stable cell-laden gelatin methacrylamide scaffolds with high cell viability (>97%) could be printed.
在本研究中，我们报告了材料化学、工程学和生物学的联合努力，作为制造高存活率 3D 打印大孔明胶甲基丙烯酰胺结构的系统方法。首先，我们提出使用和优化 VA-086 作为光引发剂，与传统的 Irgacure 2959 相比，具有增强的生物相容性。其次，对明胶的打印进行了参数研究，以对构建体结构进行特征描述和比较。在此过程中，深入分析了水凝胶构建块浓度、打印温度、打印压力、打印速度和细胞密度的影响。结果表明，在 10-20 w/v%的明胶浓度范围内，可以设计出具有 100%互连通孔网络的支架。最后一部分研究了细胞负载支架的制造，通过封装肝癌细胞系（HepG2）测试了组织工程的应用。打印压力和针形被证明会影响整体细胞活力。可以打印出具有高细胞活力（>97%）的机械稳定的细胞负载明胶甲基丙烯酰胺支架。
