Kim Jong-Geol, Park Soo-Je, Quan Zhe-Xue, Jung Man-Young, Cha In-Tae, Kim So-Jeong, Kim Kyoung-Ho, Yang Eun-Jin, Kim Young-Nam, Lee Sang-Hoon, Rhee Sung-Keun
Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Heungduk-gu, Cheongju, 361-763, South Korea.
Environ Microbiol. 2014 Jun;16(6):1566-78. doi: 10.1111/1462-2920.12287. Epub 2013 Oct 23.
Polynyas, areas of open water surrounded by sea ice, are sites of intense primary production and ecological hotspots in the Antarctic Ocean. This study determined the spatial variation in communities of prokaryotes in a polynya in the Amundsen Sea using 454 pyrosequencing technology, and the results were compared with biotic and abiotic environmental factors. The bacterial abundance was correlated with that of phytoplankton, Phaeocystis spp. and diatoms. A cluster analysis indicated that the bacterial communities in the surface waters of the polynya were distinct from those under the sea ice. Overall, two bacterial clades, Polaribacter (20-64%) and uncultivated Oceanospirillaceae (7-34%), dominated the surface water in the polynya while the Pelagibacter clade was abundant at all depths (7-42%). The archaeal communities were not as diverse as the bacterial communities in the polynya, and marine group I was dominant (> 80%). Canonical correspondence analysis indicated that the oceanographic properties facilitated the development of distinct prokaryotic assemblages in the polynya. This analysis of the diversity and composition of the psychrophilic prokaryotes associated with high phytoplankton production provides new insights into the roles of prokaryotes in biogeochemical cycles in high-latitude polynyas.
冰间湖是被海冰环绕的开阔水域，是南大洋初级生产活跃的区域和生态热点。本研究利用454焦磷酸测序技术确定了阿蒙森海中一个冰间湖中原核生物群落的空间变化，并将结果与生物和非生物环境因素进行了比较。细菌丰度与浮游植物、棕囊藻属和硅藻的丰度相关。聚类分析表明，冰间湖表层水体中的细菌群落与海冰下的不同。总体而言，两个细菌类群，极地杆菌属（20 - 64%）和未培养的海螺旋菌科（7 - 34%），在冰间湖表层水体中占主导地位，而Pelagibacter类群在所有深度都很丰富（7 - 42%）。古菌群落的多样性不如冰间湖中的细菌群落，海洋第一类群占主导地位（> 80%）。典范对应分析表明，海洋学特征促进了冰间湖中不同原核生物组合的发展。对与高浮游植物产量相关的嗜冷原核生物的多样性和组成的分析，为原核生物在高纬度冰间湖生物地球化学循环中的作用提供了新的见解。
