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蜘蛛丝蛋白的重组 DNA 生产。

Recombinant DNA production of spider silk proteins.

机构信息

Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.

出版信息

Microb Biotechnol. 2013 Nov;6(6):651-63. doi: 10.1111/1751-7915.12081.

DOI:10.1111/1751-7915.12081
PMID:24119078
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3815454/
Abstract

Spider dragline silk is considered to be the toughest biopolymer on Earth due to an extraordinary combination of strength and elasticity. Moreover, silks are biocompatible and biodegradable protein-based materials. Recent advances in genetic engineering make it possible to produce recombinant silks in heterologous hosts, opening up opportunities for large-scale production of recombinant silks for various biomedical and material science applications. We review the current strategies to produce recombinant spider silks.

摘要

蜘蛛牵引丝因其强度和弹性的非凡结合而被认为是地球上最坚韧的生物聚合物。此外，丝是生物相容和可生物降解的蛋白质基材料。基因工程的最新进展使得在异源宿主中生产重组丝成为可能，为各种生物医学和材料科学应用的重组丝的大规模生产开辟了机会。我们回顾了生产重组蜘蛛丝的当前策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/9f18c2844ab4/mbt0006-0651-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/b1a70d8cd671/mbt0006-0651-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/6a13a9a40e41/mbt0006-0651-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/d8efc017242f/mbt0006-0651-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/fd7a747b844a/mbt0006-0651-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/9f18c2844ab4/mbt0006-0651-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/b1a70d8cd671/mbt0006-0651-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/6a13a9a40e41/mbt0006-0651-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/d8efc017242f/mbt0006-0651-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/fd7a747b844a/mbt0006-0651-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8124/3815932/9f18c2844ab4/mbt0006-0651-f5.jpg

相似文献

[1]
Recombinant DNA production of spider silk proteins.

Microb Biotechnol. 2013-11

[2]
De Novo Design of Recombinant Spider Silk Proteins for Material Applications.

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[3]
Recent advances in production of recombinant spider silk proteins.

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[4]
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[5]
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[6]
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[7]
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[8]
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[9]
Engineering Circularized mRNAs for the Production of Spider Silk Proteins.

Appl Environ Microbiol. 2022-4-26

[10]
Large scale production of synthetic spider silk proteins in Escherichia coli.

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引用本文的文献

[1]
Developing the Strategy to Use Silk Spheres for Efficient, Targeted Delivery of Oligonucleotide Therapeutics to Cancer Cells.

Int J Nanomedicine. 2025-6-23

[2]
Disentangling the Web: An Interdisciplinary Review on the Potential and Feasibility of Spider Silk Bioproduction.

ACS Biomater Sci Eng. 2024-9-9

[3]
Unveiling the Dynamic Self-Assembly of a Recombinant Dragline-Silk-Mimicking Protein.

Biomacromolecules. 2024-3-11

[4]
Production and secretion of recombinant spider silk in Bacillus megaterium.

Microb Cell Fact. 2024-1-26

[5]
Factors Influencing Properties of Spider Silk Coatings and Their Interactions within a Biological Environment.

J Funct Biomater. 2023-8-19

[6]
Novel insights into construct toxicity, strain optimization, and primary sequence design for producing recombinant silk fibroin and elastin-like peptide in .

Metab Eng Commun. 2023-2-3

[7]
Protein-Based Biological Materials: Molecular Design and Artificial Production.

Chem Rev. 2023-3-8

[8]
Soft Devices for High-Resolution Neuro-Stimulation: The Interplay Between Low-Rigidity and Resolution.

Front Med Technol. 2021-6-14

[9]
Systemic and Local Silk-Based Drug Delivery Systems for Cancer Therapy.

Cancers (Basel). 2021-10-27

[10]
Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability.

Bioengineered. 2021-12

本文引用的文献

[1]
Transient Expression of a Major Ampullate Spidroin 1 Gene Fragment from sp. in Mammalian Cells.

Cancer Genomics Proteomics. 2006

[2]
Recombinant minimalist spider wrapping silk proteins capable of native-like fiber formation.

PLoS One. 2012-11-28

[3]
Engineered large spider eggcase silk protein for strong artificial fibers.

Adv Mater. 2013-2-25

[4]
Reproducing natural spider silks' copolymer behavior in synthetic silk mimics.

Biomacromolecules. 2012-11-8

[5]
Native-sized spider silk proteins synthesized in planta via intein-based multimerization.

Transgenic Res. 2012-9-22

[6]
Purification and cytotoxicity of tag-free bioengineered spider silk proteins.

J Biomed Mater Res A. 2012-8-3

[7]
Recombinant spider silk matrices for neural stem cell cultures.

Biomaterials. 2012-8-3

[8]
Recent advances in production of recombinant spider silk proteins.

Curr Opin Biotechnol. 2012-4-20

[9]
Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties.

Proc Natl Acad Sci U S A. 2012-1-3

[10]
Silk-based nanocomplexes with tumor-homing peptides for tumor-specific gene delivery.

Macromol Biosci. 2011-11-3

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